ArticleNucleic acids research2010
Measurement of replication structures at the nanometer scale using super-resolution light microscopy.
Article in Nucleic acids research, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 57 papers.
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Who cites it
57 citing papers in PubMed.
- Spatial organization and dynamics of genome replication: from forks to foci.Nucleic acids research · 2026Review
- Histone variant macroH2A1 regulates synchronous firing of replication origins in the inactive X chromosome.Nucleic acids research · 2024Article
- Super-resolved analysis of colocalization between replication and transcription along the cell cycle in a model of oncogene activation.Communications biology · 2024Article
- DNA choreography: correlating mobility and organization of DNA across different resolutions from loops to chromosomes.Histochemistry and cell biology · 2024Article
- Profiling Chromosome Topological Features by Super-Resolution 3D Structured Illumination Microscopy.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Article
- Three-dimensional structured illumination microscopy with enhanced axial resolution.Nature biotechnology · 2023Article
- Super-resolution microscopy reveals stochastic initiation of replication in Drosophila polytene chromosomes.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2022Article
- Role of Cockayne Syndrome Group B Protein in Replication Stress: Implications for Cancer Therapy.International journal of molecular sciences · 2022Review
- Replication Labeling Methods for Super-Resolution Imaging of Chromosome Territories and Chromatin Domains.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Evaluation of sted super-resolution image quality by image correlation spectroscopy (QuICS).Scientific reports · 2021Article
- A basal-level activity of ATR links replication fork surveillance and stress response.Molecular cell · 2021Article
- How Single-Molecule Localization Microscopy Expanded Our Mechanistic Understanding of RNA Polymerase II Transcription.International journal of molecular sciences · 2021Review
- Fiber-Like Organization as a Basic Principle for Euchromatin Higher-Order Structure.Frontiers in cell and developmental biology · 2021Article
- Are the processes of DNA replication and DNA repair reading a common structural chromatin unit?Nucleus (Austin, Tex.) · 2020Review
- Cohesin depleted cells rebuild functional nuclear compartments after endomitosis.Nature communications · 2020Article
- Superresolution imaging reveals spatiotemporal propagation of human replication foci mediated by CTCF-organized chromatin structures.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- RTEL1 suppresses G-quadruplex-associated R-loops at difficult-to-replicate loci in the human genome.Nature structural & molecular biology · 2020Article
- Super-Resolution Microscopy of Chromatin.Genes · 2019Review
- Single nucleosome imaging reveals loose genome chromatin networks via active RNA polymerase II.The Journal of cell biology · 2019Article
Corrections and comments
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Authors and funding
12 authors.
Funding
Abstract
DNA replication, similar to other cellular processes, occurs within dynamic macromolecular structures. Any comprehensive understanding ultimately requires quantitative data to establish and test models of genome duplication. We used two different super-resolution light microscopy techniques to directly measure and compare the size and numbers of replication foci in mammalian cells. This analysis showed that replication foci vary in size from 210 nm down to 40 nm. Remarkably, spatially modulated illumination (SMI) and 3D-structured illumination microscopy (3D-SIM) both showed an average size of 125 nm that was conserved throughout S-phase and independent of the labeling method, suggesting a basic unit of genome duplication. Interestingly, the improved optical 3D resolution identified 3- to 5-fold more distinct replication foci than previously reported. These results show that optical nanoscopy techniques enable accurate measurements of cellular structures at a level previously achieved only by electron microscopy and highlight the possibility of high-throughput, multispectral 3D analyses.
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