ArticleBMC microbiology2009
Application and evaluation of the MLVA typing assay for the Brucella abortus strains isolated in Korea.
Article in BMC microbiology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed.
- Original and introduced lineages co-driving the persistence ofFrontiers in public health · 2023Article
- Genetic variation of Brucella isolates at strain level in Egypt.Veterinary medicine and science · 2020Article
- Multilocus variable number tandem repeat analysis (MLVA)-typing of Brucella abortus isolates of India reveals limited genetic diversity.Tropical animal health and production · 2020Article
- Origins and global context of Brucella abortus in Italy.BMC microbiology · 2017Article
- Characterization of Salmonella enterica serovar Typhimurium and Salmonella enterica serovar 4,[5],12:i:- isolates from pigs presenting with diarrhea in Korea.The Journal of veterinary medical science · 2015Article
- Multiple-locus variable-number tandem-repeat analysis of Brucella isolates from patients in Xinjiang China.International journal of clinical and experimental medicine · 2015Article
- Genetic diversity of Brucella ovis isolates from Rio Grande do Sul, Brazil, by MLVA16.BMC research notes · 2014Article
- Genetic stability of Brucella abortus isolates from an outbreak by multiple-locus variable-number tandem repeat analysis (MLVA16).BMC microbiology · 2014Article
- Evaluating the impacts of stressors of Pseudomonas syringae pathovar tomato on the effectiveness of multi-locus variable number tandem repeat analysis and multi-locus sequence typing in microbial forensic investigations.Investigative genetics · 2014Article
- Brucella melitensis in France: persistence in wildlife and probable spillover from Alpine ibex to domestic animals.PloS one · 2014Article
- Molecular strain typing of Brucella abortus isolates from Italy by two VNTR allele sizing technologies.Molecular biotechnology · 2013Article
- Link between geographical origin and occurrence of Brucella abortus biovars in cow and water buffalo herds.Applied and environmental microbiology · 2013Article
- Biotyping and genotyping (MLVA16) of Brucella abortus isolated from cattle in Brazil, 1977 to 2008.PloS one · 2013Article
- MLVA genotyping of Chinese human Brucella melitensis biovar 1, 2 and 3 isolates.BMC microbiology · 2011Article
- Genotyping analysis of Helicobacter pylori using multiple-locus variable-number tandem-repeats analysis in five regions of China and Japan.BMC microbiology · 2011Article
- Epidemiological and phylogenetic analysis of Spanish human Brucella melitensis strains by multiple-locus variable-number tandem-repeat typing, hypervariable octameric oligonucleotide fingerprinting, and rpoB typing.Journal of clinical microbiology · 2010Article
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8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundA Brucella eradication program has been executed in Korea. To effectively prevent and control brucellosis, a molecular method for genetic identification and epidemiological trace-back must be established. As part of that, the MLVA typing assay was evaluated and applied to B. abortus isolates for analyzing the characteristics of the regional distribution and relationships of foreign isolates.
resultsA total of 177 isolates originating from 105 cattle farms for the period 1996 to 2008 were selected as representatives for the nine provinces of South Korea. A dendrogram of strain relatedness was constructed in accordance with the number of tandem repeat units for 17 loci so that it was possible to trace back in the restricted areas. Even in a farm contaminated by one source, however, the Brucella isolates showed an increase or decrease in one TRs copy number at some loci with high DI values. Moreover, those 17 loci was confirmed in stability via in-vitro and in-vivo passage, and found to be sufficiently stable markers that can readily identify the inoculated strain even if minor changes were detected. In the parsimony analysis with foreign Brucella isolates, domestic isolates were clustered distinctively, and located near the Central and Southern American isolates.
conclusionThe MLVA assay has enough discrimination power in the Brucella species level and can be utilized as a tool for the epidemiological trace-back of the B. abortus isolates. But it is important to consider that Brucella isolates may be capable of undergoing minor changes at some loci in the course of infection or in accordance with the changes of the host.
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