Trial reportTransgenic research2010
Germ-line transmission of lentiviral PGK-EGFP integrants in transgenic cattle: new perspectives for experimental embryology.
Trial report in Transgenic research, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
14 citing papers in PubMed, 30 citations in OpenAlex.
- Long-term (10-year) monitoring of transposon-mediated transgenic cattle.Transgenic research · 2024Article
- Prenatal Somatic Cell Gene Therapies: Charting a Path Toward Clinical Applications (Proceedings of the CERSI-FDA Meeting).Journal of clinical pharmacology · 2022Review
- A New Toolbox in Experimental Embryology-Alternative Model Organisms for Studying Preimplantation Development.Journal of developmental biology · 2021Review
- Assisted Reproductive Techniques and Genetic Manipulation in the Common Marmoset.ILAR journal · 2020Review
- Long-term health and germline transmission in transgenic cattle following transposon-mediated gene transfer.BMC genomics · 2018Article
- OCT4/POU5F1 is required for NANOG expression in bovine blastocysts.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- Single-cell RNA sequencing reveals developmental heterogeneity of blastomeres during major genome activation in bovine embryos.Scientific reports · 2018Article
- Role of miR-155 in the regulation of MMP-16 expression in intervertebral disc degeneration.Journal of orthopaedic research : official publication of the Orthopaedic Research Society · 2017Article
- Efficient generation of transgenic cattle using the DNA transposon and their analysis by next-generation sequencing.Scientific reports · 2016Article
- Generation of transgenic cynomolgus monkeys that express green fluorescent protein throughout the whole body.Scientific reports · 2016Article
- Embryo development, fetal growth and postnatal phenotype of eGFP lambs generated by lentiviral transgenesis.Transgenic research · 2015Article
- [Advances of lentiviral vectors].Zhongguo fei ai za zhi = Chinese journal of lung cancer · 2014Review
- Highly efficient generation of transgenic sheep by lentivirus accompanying the alteration of methylation status.PloS one · 2013Article
- Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
13 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Lentiviral vectors are a powerful tool for the genetic modification of livestock species. We previously generated transgenic founder cattle with lentiviral integrants carrying enhanced green fluorescent protein (EGFP) under the control of the phosphoglycerate kinase (PGK) promoter. In this study, we investigated the transmission of LV-PGK-EGFP integrants through the female and male germ line in cattle. A transgenic founder heifer (#562, Kiki) was subjected to superovulation treatment and inseminated with semen from a non-transgenic bull. Embryos were recovered and transferred to synchronized recipient heifers, resulting in the birth of a healthy male transgenic calf expressing EGFP as detected by in vivo imaging. Semen from a transgenic founder bull (#561, Jojo) was used for in vitro fertilization (IVF) of in vitro matured (IVM) oocytes from non-transgenic cows. The rates of cleavage and development to blastocyst in vitro corresponded to 52.0 +/- 4.1 and 24.5 +/- 4.4%, respectively. Expression of EGFP was observed at blastocyst stage (day 7 after IVF) and was seen in 93.0% (281/302) of the embryos. 24 EGFP-expressing embryos were transferred to 9 synchronized recipients. Analysis of 2 embryos, flushed from the uterus on day 15, two fetuses recovered on day 45, and a healthy male transgenic calf revealed consistent high-level expression of EGFP in all tissues investigated. Our study shows for the first time transmission of lentiviral integrants through the germ line of female and male transgenic founder cattle. The pattern of inheritance was consistent with Mendelian rules. Importantly, high fidelity expression of EGFP in embryos, fetuses, and offspring of founder #561 provides interesting tools for developmental studies in cattle, including interactions of gametes, embryos and fetuses with their maternal environment.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.