ArticleMolecular and cellular biology1991
Characterization of a short, cis-acting DNA sequence which conveys cell cycle stage-dependent transcription in Saccharomyces cerevisiae.
Article in Molecular and cellular biology, 1991. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 52 papers.
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Who cites it
52 citing papers in PubMed, 117 citations in OpenAlex.
- Investigating Conservation of the Cell-Cycle-Regulated Transcriptional Program in the Fungal Pathogen, Cryptococcus neoformans.PLoS genetics · 2016Article
- The Forkhead transcription factor Hcm1 regulates chromosome segregation genes and fills the S-phase gap in the transcriptional circuitry of the cell cycle.Genes & development · 2006Article
- Article
- MCB-mediated regulation of cell cycle-specific cdc22+ transcription in fission yeast.Molecular genetics and genomics : MGG · 2003Article
- Mps3p is a novel component of the yeast spindle pole body that interacts with the yeast centrin homologue Cdc31p.The Journal of cell biology · 2002Article
- Determining binding sites in protein-nucleic acid complexes by cross-saturation.Journal of biomolecular NMR · 2001Article
- The influence of DNA binding on the backbone dynamics of the yeast cell-cycle protein Mbp1.Journal of biomolecular NMR · 2000Article
- Sli15 associates with the ipl1 protein kinase to promote proper chromosome segregation in Saccharomyces cerevisiae.The Journal of cell biology · 1999Article
- Article
- Comprehensive identification of cell cycle-regulated genes of the yeast Saccharomyces cerevisiae by microarray hybridization.Molecular biology of the cell · 1998Article
- Regulation of Cdc28 cyclin-dependent protein kinase activity during the cell cycle of the yeast Saccharomyces cerevisiae.Microbiology and molecular biology reviews : MMBR · 1998Review
- Functional domains of rep2, a transcriptional activator subunit for Res2-Cdc10, controlling the cell cycle "start".Molecular biology of the cell · 1998Article
- Article
- A small conserved domain in the yeast Spa2p is necessary and sufficient for its polarized localization.The Journal of cell biology · 1997Article
- Role of the casein kinase I isoform, Hrr25, and the cell cycle-regulatory transcription factor, SBF, in the transcriptional response to DNA damage in Saccharomyces cerevisiae.Proceedings of the National Academy of Sciences of the United States of America · 1997Article
- Domains determining the functional distinction of the fission yeast cell cycle "start" molecules Res1 and Res2.Molecular biology of the cell · 1996Article
- Transcription of mutS and mutL-homologous genes in Saccharomyces cerevisiae during the cell cycle.Molecular & general genetics : MGG · 1996Article
- Analysis of Tub4p, a yeast gamma-tubulin-like protein: implications for microtubule-organizing center function.The Journal of cell biology · 1996Article
- Pds1p is required for faithful execution of anaphase in the yeast, Saccharomyces cerevisiae.The Journal of cell biology · 1996Article
- Spc42p: a phosphorylated component of the S. cerevisiae spindle pole body (SPD) with an essential function during SPB duplication.The Journal of cell biology · 1996Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Comparison of the 5'-flanking regions of several cell cycle-regulated DNA replication genes of Saccharomyces cerevisiae has revealed the presence of a common sequence, 5'-ACGCGT-3', which is upstream and proximal to mapped transcription initiation sites. This sequence, which is the cleavage site for the restriction endonuclease MluI, is present twice in the upstream region of the yeast thymidylate synthase gene TMP1. Previous studies have implicated these MluI sites as critical components in the cell cycle-dependent transcription of TMP1. In this study, we examined more closely the importance of the ACGCGT sequences for the transcription of this gene. Using site-directed mutagenesis in combination with deletion analysis and subcloning experiments, we found that (i) while both of the TMP1 MluI sites contribute to the total transcription of this gene, the distal site is predominant and (ii) the 9-bp sequence ACGCGTTAA encompassing the distal MluI site exhibits properties of a cell cycle-stage dependent upstream activation sequence element. The results of this study support the notion that the ACGCGT sequence is an integral component of a transcription system which coordinates the cell cycle-dependent expression of DNA replication genes in S. cerevisiae.
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