ArticleJournal of experimental botany2010
Three cotton genes preferentially expressed in flower tissues encode actin-depolymerizing factors which are involved in F-actin dynamics in cells.
Article in Journal of experimental botany, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 42 citations in OpenAlex.
- Understanding the heat stress mitigation mechanisms during pollen development in Gossypium hirsutum L.Molecular biology reports · 2026Article
- Functional Characterization of the SoybeanPlants (Basel, Switzerland) · 2024Article
- Evolution of the thermostability of actin-depolymerizing factors enhances the adaptation of pollen germination to high temperature.The Plant cell · 2024Article
- A comprehensive overview of cotton genomics, biotechnology and molecular biological studies.Science China. Life sciences · 2023Review
- Research progress on the roles of actin-depolymerizing factor in plant stress responses.Frontiers in plant science · 2023Review
- Pedigree-based QTL analysis of flower size traits in two multi-parental diploid rose populations.Frontiers in plant science · 2023Article
- GhADF6-mediated actin reorganization is associated with defence against Verticillium dahliae infection in cotton.Molecular plant pathology · 2021Article
- Hyperoside promotes pollen tube growth by regulating the depolymerization effect of actin-depolymerizing factor 1 on microfilaments in okra.Horticulture research · 2021Article
- Genome-Wide Identification and Low Temperature Responsive Pattern of Actin Depolymerizing Factor (ADF) Gene Family in Wheat (Frontiers in plant science · 2021Article
- Article
- An actin-depolymerizing factor from the halophyte smooth cordgrass, Spartina alterniflora (SaADF2), is superior to its rice homolog (OsADF2) in conferring drought and salt tolerance when constitutively overexpressed in rice.Plant biotechnology journal · 2019Article
- Genetic Architecture of a Rice Nested Association Mapping Population.G3 (Bethesda, Md.) · 2017Article
- Article
- Arabidopsis actin-depolymerizing factor7 severs actin filaments and regulates actin cable turnover to promote normal pollen tube growth.The Plant cell · 2013Article
- Transcriptomic analysis of differentially expressed genes during anther development in genetic male sterile and wild type cotton by digital gene-expression profiling.BMC genomics · 2013Article
- Comprehensive analysis of differentially expressed rice actin depolymerizing factor gene family and heterologous overexpression of OsADF3 confers Arabidopsis Thaliana drought tolerance.Rice (New York, N.Y.) · 2012Article
- A molecular and proteomic investigation of proteins rapidly released from triticale pollen upon hydration.Plant molecular biology · 2012Article
- Generation, annotation and analysis of first large-scale expressed sequence tags from developing fiber of Gossypium barbadense L.PloS one · 2011Article
Corrections and comments
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
To investigate whether the high expression levels of actin-depolymerizing factor genes are related to pollen development, three GhADF genes (cDNAs) were isolated and characterized in cotton. Among them, GhADF6 and GhADF8 were preferentially expressed in petals, whereas GhADF7 displayed the highest level of expression in anthers, revealing its anther specificity. The GhADF7 transcripts in anthers reached its peak value at flowering, suggesting that its expression is developmentally-regulated in anthers. The GhADF7 gene including the promoter region was isolated from the cotton genome. To demonstrate the specificity of the GhADF7 promoter, the 5'-flanking region, including the promoter and 5'-untranslated region, was fused with the GUS gene. Histochemical assays demonstrated that the GhADF7:GUS gene was specifically expressed in pollen grains. When pollen grains germinated, very strong GUS staining was detected in the elongating pollen tube. Furthermore, overexpression of GhADF7 gene in Arabidopsis thaliana reduced the viable pollen grains and, consequently, transgenic plants were partially male-sterile. Overexpression of GhADF7 in fission yeast (Schizosaccharomyces pombe) altered the balance of actin depolymerization and polymerization, leading to the defective cytokinesis and multinucleate formation in the cells. Given all the above results together, it is proposed that the GhADF7 gene may play an important role in pollen development and germination.
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