ArticleVirology2010
Nucleolin/Nsr1p binds to the 3' noncoding region of the tombusvirus RNA and inhibits replication.
Article in Virology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
22 citing papers in PubMed, 38 citations in OpenAlex.
- Mobilization of nuclear antiviral factors by exportin XPO1 via the actin network inhibits RNA virus replication.PLoS pathogens · 2025Article
- Host protein chaperones, RNA helicases and the ubiquitin network highlight the arms race for resources between tombusviruses and their hosts.Advances in virus research · 2020Article
- Nucleolin mediates the internalization of rabbit hemorrhagic disease virus through clathrin-dependent endocytosis.PLoS pathogens · 2018Article
- Article
- Functions of the 5' and 3' ends of calicivirus genomes.Virus research · 2015Review
- The hop-like stress-induced protein 1 cochaperone is a novel cell-intrinsic restriction factor for mitochondrial tombusvirus replication.Journal of virology · 2014Article
- Viruses and the nucleolus: the fatal attraction.Biochimica et biophysica acta · 2014Article
- Article
- Tombusvirus-yeast interactions identify conserved cell-intrinsic viral restriction factors.Frontiers in plant science · 2014Review
- Cyclophilin A binds to the viral RNA and replication proteins, resulting in inhibition of tombusviral replicase assembly.Journal of virology · 2013Article
- Nucleolin interacts with the dengue virus capsid protein and plays a role in formation of infectious virus particles.Journal of virology · 2013Article
- Identification of novel host factors via conserved domain search: Cns1 cochaperone is a novel restriction factor of tombusvirus replication in yeast.Journal of virology · 2013Article
- The glutathione transferase of Nicotiana benthamiana NbGSTU4 plays a role in regulating the early replication of Bamboo mosaic virus.The New phytologist · 2013Article
- Authentic in vitro replication of two tombusviruses in isolated mitochondrial and endoplasmic reticulum membranes.Journal of virology · 2012Article
- Proteome-wide overexpression of host proteins for identification of factors affecting tombusvirus RNA replication: an inhibitory role of protein kinase C.Journal of virology · 2012Article
- The TPR domain in the host Cyp40-like cyclophilin binds to the viral replication protein and inhibits the assembly of the tombusviral replicase.PLoS pathogens · 2012Article
- Garlic virus X 11-kDa protein granules move within the cytoplasm and traffic a host protein normally found in the nucleolus.Molecular plant pathology · 2011Article
- Nucleolin interacts with the feline calicivirus 3' untranslated region and the protease-polymerase NS6 and NS7 proteins, playing a role in virus replication.Journal of virology · 2011Article
- Global genomics and proteomics approaches to identify host factors as targets to induce resistance against Tomato bushy stunt virus.Advances in virus research · 2010Review
- Involvement of the plant nucleolus in virus and viroid infections: parallels with animal pathosystems.Advances in virus research · 2010Review
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Previous genome-wide screens identified >100 host genes affecting tombusvirus replication using yeast model host. One of those factors was Nsr1p (nucleolin), which is an abundant RNA-binding shuttle protein involved in rRNA maturation and ribosome assembly. We find that overexpression of Nsr1p in yeast or in Nicotiana benthamiana inhibited the accumulation of tombusvirus RNA by approximately 10-fold. Regulated overexpression of Nsr1p revealed that Nsr1p should be present at the beginning of viral replication for efficient inhibition, suggesting that Nsr1p inhibits an early step in the replication process. In vitro experiments revealed that Nsr1p binds preferably to the 3' UTR in the viral RNA. The purified recombinant Nsr1p inhibited the in vitro replication of the viral RNA in a yeast cell-free assay when preincubated with the viral RNA before the assay. These data support the model that Nsr1p/nucleolin inhibits tombusvirus replication by interfering with the recruitment of the viral RNA for replication.
Indexed as
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.