ArticleReproductive biology and endocrinology : RB&E2009
The BSA-induced Ca2+ influx during sperm capacitation is CATSPER channel-dependent.
Article in Reproductive biology and endocrinology : RB&E, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 51 papers, 1 of them a synthesis that pooled it.
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Who cites it
51 citing papers in PubMed, 1 synthesis or guideline pooled it, 125 citations in OpenAlex.
- The Catsper channel and its roles in male fertility: a systematic review.Reproductive biology and endocrinology : RB&E · 2017Pooled it
- Regulation of spermatozoal capacitation by serotonin.The Journal of reproduction and development · 2026Review
- Tryptophan promotes sperm hyperactivation in hamsters via 5-hydroxytryptamine biosynthesis within sperm.The Journal of reproduction and development · 2025Article
- Time and Media-Dependent Modulation of Sperm Capacitation in Red-Rumped Agouti (Dasyprocta leporina Linnaeus, 1758).Molecular reproduction and development · 2025Article
- Bovine serum albumin-induced calcium influx triggers soluble adenylyl cyclase activation and cyclic AMP signalling pathways in mouse sperm capacitation.The Journal of physiology · 2025Article
- Influences of 5-hydroxytriptamine on sperm hyperactivation and in vitro fertility in rats.The Journal of reproduction and development · 2025Article
- Progesterone and estradiol regulate sperm hyperactivation and in vitro fertilization success in mice.The Journal of reproduction and development · 2024Article
- In vivo CRISPR screening directly targeting testicular cells.Cell genomics · 2024Article
- Differential role of bovine serum albumin and HCO3- in the regulation of GSK3 alpha during mouse sperm capacitation.Molecular human reproduction · 2024Article
- Enhancement of rat spermatozoal hyperactivation by progesterone.The Journal of reproduction and development · 2023Article
- The Chemosensing Role of CatSper in Mammalian Sperm: An Updated Review.Current issues in molecular biology · 2023Review
- Progesterone increases the success of in vitro fertilization via enhanced sperm hyperactivation in mice.The Journal of reproduction and development · 2023Article
- CatSper Calcium Channels: 20 Years On.Physiology (Bethesda, Md.) · 2023Review
- In Vitro versus Cryo-Induced Capacitation of Bovine Spermatozoa, Part 2: Changes in the Expression Patterns of Selected Transmembrane Channels and Protein Kinase A.International journal of molecular sciences · 2022Article
- 3D structure and in situ arrangements of CatSper channel in the sperm flagellum.Nature communications · 2022Article
- C2CD6 regulates targeting and organization of the CatSper calcium channel complex in sperm flagella.Development (Cambridge, England) · 2022Article
- Bundle formation of sperm: Influence of environmental factors.Frontiers in endocrinology · 2022Article
- Exogenous Albumin Is Crucial for Pig Sperm to Elicit In Vitro Capacitation Whereas Bicarbonate Only Modulates Its Efficiency.Biology · 2021Article
- Uncovering the signalling, structure and function of the 20-HETE-GPR75 pairing: Identifying the chemokine CCL5 as a negative regulator of GPR75.British journal of pharmacology · 2021Article
- Serotonergic signals enhanced hamster sperm hyperactivation.The Journal of reproduction and development · 2021Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
backgroundSerum albumin is a key component in mammalian sperm capacitation, a functional maturation process by which sperm become competent to fertilize oocytes. Capacitation is accompanied by several cellular and molecular changes including an increased tyrosine phosphorylation of sperm proteins and a development of hyperactivated sperm motility. Both of these processes require extracellular calcium, but how calcium enters sperm during capacitation is not well understood.
methodsBSA-induced changes in intracellular calcium concentration were studied using Fluo-4 and Fura-2 calcium imaging with wild-type and Catsper1 knockout mouse sperm.
resultsWe found that the fast phase of the BSA-induced rises in intracellular calcium concentration was absent in the Catsper1 knockout sperm and could be restored by an EGFP-CATSPER1 fusion protein. The calcium concentration increases were independent of G-proteins and phospholipase C but could be partially inhibited when intracellular pH was clamped. The changes started in the principal piece and propagated toward the sperm head.
conclusionWe conclude that the initial phase of the increases in intracellular calcium concentration induced by BSA requires the CATSPER channel, but not the voltage-gated calcium channel. Our findings identify the molecular conduit responsible for the calcium entry required for the sperm motility changes that occur during capacitation.
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