ArticleThe journal of physical chemistry. B2010
Azido push-pull fluorogens photoactivate to produce bright fluorescent labels.
Article in The journal of physical chemistry. B, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed.
- Article
- Azidocoumarin Glycan Probes for Photoinduced Cross-Linking and In Situ Fluorescent Labeling.Bioconjugate chemistry · 2026Article
- A Fluorogenic Reaction for Monitoring Cross-Coupling with Turn-On Ratio Greater than a Hundred Thousand.Chemical & biomedical imaging · 2026Article
- Small Molecule Optical Probes for Detection of HACS omega · 2024Review
- Maltose-Derivatized Fluorescence Turn-On Imaging Probe for Bacteria Detection.ACS infectious diseases · 2023Article
- A Photoalkylative Fluorogenic Probe of Guttiferone A for Live Cell Imaging and Proteome Labeling inMolecules (Basel, Switzerland) · 2020Article
- Super-resolution Microscopy with Single Molecules in Biology and Beyond-Essentials, Current Trends, and Future Challenges.Journal of the American Chemical Society · 2020Review
- Clickable azide-functionalized bromoarylaldehydes - synthesis and photophysical characterization.Beilstein journal of organic chemistry · 2020Article
- Ultraspecific live imaging of the dynamics of zebrafish neutrophil granules by a histopermeable fluorogenic benzochalcone probe.Chemical science · 2019Article
- A Cysteine-Reactive Small Photo-Crosslinker Possessing Caged-Fluorescence Properties: Binding-Site Determination of a Combinatorially-Selected Peptide by Fluorescence Imaging/Tandem Mass Spectrometry.International journal of molecular sciences · 2018Article
- Switchable Fluorophores for Single-Molecule Localization Microscopy.Chemical reviews · 2018Review
- Diversity-Oriented Synthesis and Optical Properties of Bichromophoric Pyrrole-Fluorophore Conjugates.Frontiers in chemistry · 2018Article
- Photoactivatable fluorescein derivatives caged with a (3-hydroxy-2-naphthalenyl)methyl group.The Journal of organic chemistry · 2014Article
- Article
- An unexpected copper catalyzed 'reduction' of an arylazide to amine through the formation of a nitrene intermediate.Tetrahedron · 2013Article
- Photoremovable protecting groups in chemistry and biology: reaction mechanisms and efficacy.Chemical reviews · 2013Review
- A general approach to break the concentration barrier in single-molecule imaging.Nature methods · 2012Article
- Two-photon activated two-photon fluorescence and binding of azidocoumarin in a gelatin matrix.Journal of fluorescence · 2012Article
- Optimal 3D single-molecule localization for superresolution microscopy with aberrations and engineered point spread functions.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- Multicolor super-resolution fluorescence imaging via multi-parameter fluorophore detection.Chemphyschem : a European journal of chemical physics and physical chemistry · 2012Article
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Authors and funding
9 authors.
Funding
Abstract
Dark azido push-pull chromophores have the ability to be photoactivated to produce bright fluorescent labels suitable for single-molecule imaging. Upon illumination, the aryl azide functionality in the fluorogens participates in a photochemical conversion to an aryl amine, thus restoring charge-transfer absorption and fluorescence. Previously, we reported that one compound, DCDHF-V-P-azide, was photoactivatable. Here, we demonstrate that the azide-to-amine photoactivation process is generally applicable to a variety of push-pull chromophores, and we characterize the photophysical parameters including photoconversion quantum yield, photostability, and turn-on ratio. Azido push-pull fluorogens provide a new class of photoactivatable single-molecule probes for fluorescent labeling and super-resolution microscopy. Lastly, we demonstrate that photoactivated push-pull dyes can insert into bonds of nearby biomolecules, simultaneously forming a covalent bond and becoming fluorescent (fluorogenic photoaffinity labeling).
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.