ArticleThe Journal of cell biology1991
Reconstitution of constitutive secretion using semi-intact cells: regulation by GTP but not calcium.
Article in The Journal of cell biology, 1991. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 47 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
47 citing papers in PubMed, 112 citations in OpenAlex.
- CaBMC biology · 2025Article
- TRIM21 and PHLDA3 negatively regulate the crosstalk between the PI3K/AKT pathway and PPP metabolism.Nature communications · 2020Article
- Intracellular Delivery by Membrane Disruption: Mechanisms, Strategies, and Concepts.Chemical reviews · 2018Review
- Astrocyte VAMP3 vesicles undergo Ca2+ -independent cycling and modulate glutamate transporter trafficking.The Journal of physiology · 2015Article
- NAADP/TPC2/Ca(2+) Signaling Inhibits Autophagy.Communicative & integrative biology · 2013Article
- Trafficking of the NMDAR2B receptor subunit distal cytoplasmic tail from endoplasmic reticulum to the synapse.PloS one · 2012Article
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- Munc13-2-/- baseline secretion defect reveals source of oligomeric mucins in mouse airways.The Journal of physiology · 2008Article
- Modification of a hydrophobic layer by a point mutation in syntaxin 1A regulates the rate of synaptic vesicle fusion.PLoS biology · 2007Article
- Imaging constitutive exocytosis with total internal reflection fluorescence microscopy.The Journal of cell biology · 2000Article
- ARF6 targets recycling vesicles to the plasma membrane: insights from an ultrastructural investigation.The Journal of cell biology · 1998Article
- Plasma membrane localization of G alpha z requires two signals.Molecular biology of the cell · 1998Article
- Internucleosomal DNA cleavage triggered by plasma membrane damage during necrotic cell death. Involvement of serine but not cysteine proteases.The American journal of pathology · 1997Article
- Ca2+-independent and Ca2+/GTP-binding protein-controlled exocytosis in a plant cell.Proceedings of the National Academy of Sciences of the United States of America · 1997Article
- Lysosomes behave as Ca2+-regulated exocytic vesicles in fibroblasts and epithelial cells.The Journal of cell biology · 1997Article
- Protein kinase C mu is located at the Golgi compartment.The Journal of cell biology · 1996Article
- Ca2+ triggers massive exocytosis in Chinese hamster ovary cells.The EMBO journal · 1996Article
- Real-time detection of the surface delivery of newly synthesized membrane proteins.Proceedings of the National Academy of Sciences of the United States of America · 1996Article
- Feedback control of milk secretion from milk.Journal of mammary gland biology and neoplasia · 1996Review
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Regulated exocytosis in many permeabilized cells can be triggered by calcium and nonhydrolyzable GTP analogues. Here we examine the role of these effectors in exocytosis of constitutive vesicles using a system that reconstitutes transport between the trans-Golgi region and the plasma membrane. Transport is assayed by two independent methods: the movement of a transmembrane glycoprotein (vesicular stomatitis virus glycoprotein [VSV G protein]) to the cell surface; and the release of a soluble marker, sulfated glycosaminoglycan (GAG) chains, that have been synthesized and radiolabeled in the trans-Golgi. The plasma membrane of CHO cells was selectively perforated with the bacterial cytolysin streptolysin-O. These perforated cells allow exchange of ions and cytosolic proteins but retain intracellular organelles and transport vesicles. Incubation of the semi-intact cells with ATP and a cytosolic fraction results in transport of VSV G protein and GAG chains to the cell surface. The transport reaction is temperature dependent, requires hydrolyzable ATP, and is inhibited by N-ethylmaleimide. Nonhydrolyzable GTP analogs such as GTP gamma S, which stimulate the fusion of regulated secretory granules, completely abolish constitutive secretion. The rate and extent of constitutive transport between the trans-Golgi and the plasma membrane is independent of free Ca2+ concentrations. This is in marked contrast to fusion of regulated secretory granules with the plasma membrane, and transport between the ER and the cis-Golgi (Beckers, C. J. M., and W. E. Balch. 1989. J. Cell Biol. 108:1245-1256; Baker, D., L. Wuestehube, R. Schekman, and D. Botstein. 1990. Proc. Natl. Acad. Sci. USA. 87:355-359).
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.