ArticlePloS one2009
Proteomic and phospho-proteomic profile of human platelets in basal, resting state: insights into integrin signaling.
Article in PloS one, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 61 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
61 citing papers in PubMed, 1 synthesis or guideline pooled it, 149 citations in OpenAlex.
- Effectiveness of platelet-rich plasma in the management of hip osteoarthritis: a systematic review and meta-analysis.Clinical rheumatology · 2021Pooled it
- Rasagiline for amyotrophic lateral sclerosis: A randomized, controlled trial.Muscle & nerve · 2019Trial
- Temporal phosphoproteomics reveals key regulators of procoagulant COAT platelet generation.Blood vessels, thrombosis & hemostasis · 2026Article
- Diminished Ovarian Reserve: A Narrative Review of Etiologies and Possible Therapeutic Approaches.Journal of menopausal medicine · 2025Review
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- Association between platelet-to-high-density lipoprotein cholesterol ratio and cognitive function in older americans: insights from a cross-sectional study.Scientific reports · 2024Article
- Article
- Effect of intrauterine infusion of autologous platelet-rich plasma in patients with refractory thin endometrium undergoing in vitro fertilization.Przeglad menopauzalny = Menopause review · 2023Article
- Article
- Elevated serum platelet count inhibits the effects of brain functional changes on cognitive function in patients with mild cognitive impairment: A resting-state functional magnetic resonance imaging study.Frontiers in aging neuroscience · 2023Article
- A New Role of NAP1L1 in Megakaryocytes and Human Platelets.International journal of molecular sciences · 2022Article
- The effect of EMPAgliflozin on markers of inflammation in patients with concomitant type 2 diabetes mellitus and Coronary ARtery Disease: the EMPA-CARD randomized controlled trial.Diabetology & metabolic syndrome · 2022Article
- Umbilical Cord PRP vs. Autologous PRP for the Treatment of Hip Osteoarthritis.Journal of clinical medicine · 2022Article
- Nanoplateletsomes restrain metastatic tumor formation through decoy and active targeting in a preclinical mouse model.Acta pharmaceutica Sinica. B · 2022Article
- VEGF-attenuated platelet-rich plasma improves therapeutic effect on cartilage repair.Biomaterials science · 2022Article
- Progress of Platelet Derivatives for Cartilage Tissue Engineering.Frontiers in bioengineering and biotechnology · 2022Review
- Impact of platelet-rich plasma versus selenium in ameliorating induced toxicity in rat testis: histological, immunohistochemical, and molecular study.Cell and tissue research · 2021Article
- Circulating platelets as liquid biopsy sources for cancer detection.Molecular oncology · 2021Review
- Human Mesenchymal Stem Cell Therapy and Other Novel Treatment Approaches for Premature Ovarian Insufficiency.Reproductive sciences (Thousand Oaks, Calif.) · 2021Review
- Article
1 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
12 authors at 6 institutions in 1 country.
Funding
Abstract
During atherogenesis and vascular inflammation quiescent platelets are activated to increase the surface expression and ligand affinity of the integrin alphaIIbbeta3 via inside-out signaling. Diverse signals such as thrombin, ADP and epinephrine transduce signals through their respective GPCRs to activate protein kinases that ultimately lead to the phosphorylation of the cytoplasmic tail of the integrin alphaIIbbeta3 and augment its function. The signaling pathways that transmit signals from the GPCR to the cytosolic domain of the integrin are not well defined. In an effort to better understand these pathways, we employed a combination of proteomic profiling and computational analyses of isolated human platelets. We analyzed ten independent human samples and identified a total of 1507 unique proteins in platelets. This is the most comprehensive platelet proteome assembled to date and includes 190 membrane-associated and 262 phosphorylated proteins, which were identified via independent proteomic and phospho-proteomic profiling. We used this proteomic dataset to create a platelet protein-protein interaction (PPI) network and applied novel contextual information about the phosphorylation step to introduce limited directionality in the PPI graph. This newly developed contextual PPI network computationally recapitulated an integrin signaling pathway. Most importantly, our approach not only provided insights into the mechanism of integrin alphaIIbbeta3 activation in resting platelets but also provides an improved model for analysis and discovery of PPI dynamics and signaling pathways in the future.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.