ArticleInfection and immunity2010
Systematic analysis of the SsrAB virulon of Salmonella enterica.
Article in Infection and immunity, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 44 papers.
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Who cites it
44 citing papers in PubMed.
- Optimized cumate toolkit for tunable protein expression duringApplied and environmental microbiology · 2026Article
- Engineered bacteria launch and control an oncolytic virus.Nature biomedical engineering · 2026Article
- Deciphering the role of SsaE in biofilm regulation and T3SS-dependent virulence of Salmonella Typhimurium.World journal of microbiology & biotechnology · 2025Article
- Multifaceted Evolution of the PhoPQ Two-Component System in Salmonella enterica Enhanced the Expression of Horizontally Acquired Virulence Genes.Molecular microbiology · 2025Article
- Comparative Patho-Genomics ofPathogens (Basel, Switzerland) · 2025Article
- ChIP-mini: a low-input ChIP-exo protocol for elucidating DNA-binding protein dynamics in intracellular pathogens.Nucleic acids research · 2025Article
- Hierarchic regulation of a metabolic pathway: H-NS, CRP, and SsrB controlMicrobiology spectrum · 2024Article
- Evolutionary barriers to horizontal gene transfer in macrophage-associatedEvolution letters · 2023Article
- Salmonella T3SS effector SseK1 arginine-glycosylates the two-component response regulator OmpR to alter bile salt resistance.Scientific reports · 2023Article
- Evaluation ofVaccines · 2022Article
- The NEL Family of Bacterial E3 Ubiquitin Ligases.International journal of molecular sciences · 2022Review
- Recent Advancements in Tracking Bacterial Effector Protein Translocation.Microorganisms · 2022Review
- Transcriptome Analyses of Chicken Primary Macrophages Infected With AttenuatedFrontiers in microbiology · 2022Article
- Secretory System Components as Potential Prophylactic Targets for Bacterial Pathogens.Biomolecules · 2021Review
- Proteomics of intracellular Salmonella enterica reveals roles of Salmonella pathogenicity island 2 in metabolism and antioxidant defense.PLoS pathogens · 2019Article
- Role of CsrA in stress responses and metabolism important for Salmonella virulence revealed by integrated transcriptomics.PloS one · 2019Article
- Identification of Genetic Features for Attenuation of TwoFrontiers in veterinary science · 2019Article
- The small RNA RssR regulates myo-inositol degradation by Salmonella enterica.Scientific reports · 2018Article
- Guanosine tetraphosphate relieves the negative regulation of Salmonella pathogenicity island-2 gene transcription exerted by the AT-rich ssrA discriminator region.Scientific reports · 2018Article
- Optimized Fluorescence Complementation Platform for Visualizing Salmonella Effector Proteins Reveals Distinctly Different Intracellular Niches in Different Cell Types.ACS infectious diseases · 2017Article
Corrections and comments
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Authors and funding
2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Intracellular Salmonella enterica serovar Typhimurium deploys the Salmonella pathogenicity island 2 (SPI2)-encoded type III secretion system (T3SS) to modify host cell functions and accomplish intracellular replication. This virulence function is controlled by the two-component system SsrAB that regulates the expression of several operons in SPI2 and, in addition, a large number of genes for non-SPI2-encoded effector proteins. Here, we analyzed the relative expression levels of members of the SsrAB virulon. We used a novel reporter fusion strategy for single-copy chromosomal fusions, all done in an identical manner in order to enable direct quantitative comparison. We observed very high expression levels for sseJ and sifA; high expression levels for ssaG, steC, sseL, and sopD2; moderate expression levels for ssaB, sseA, sseG, sifB, pipB2, and sspH1; and low expression levels for sspH2, sseI, slrP, sseK1, sseK2, pipB, and gogB. The expression of the SsrAB virulon was highly dependent on the function of SsrB but also required EnvR/OmpZ. Deletion of PhoP, part of the global regulatory system PhoPQ, resulted in highly delayed expression of the SsrAB virulon under in vitro conditions; however, maximal expression was similar to that in a wild-type background. The expression levels of SsrAB-dependent genes in intracellular bacteria were in good agreement with in vitro analyses. We provide here a comprehensive and fully comparable analysis of the expression of genes in the SsrAB virulon. This information will be of interest for the selection of in vivo-activated promoters, for example, for rational design of recombinant vaccines.
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