ArticleAntimicrobial agents and chemotherapy2010
Phim46.1, the main Streptococcus pyogenes element carrying mef(A) and tet(O) genes.
Article in Antimicrobial agents and chemotherapy, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 47 papers.
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Who cites it
47 citing papers in PubMed, 80 citations in OpenAlex.
- Global spread of Streptococcus pyogenes A genomics-supported narrative review.FEMS microbiology reviews · 2025Review
- Mechanisms of bacterial immunity, protection, and survival during interbacterial warfare.Cell host & microbe · 2024Review
- TheJournal of bacteriology · 2023Article
- Conjugative transfer of streptococcal prophages harboring antibiotic resistance and virulence genes.The ISME journal · 2023Article
- Various Mobile Genetic Elements Involved in the Dissemination of the Phenicol-Oxazolidinone Resistance GeneMicrobiology spectrum · 2023Article
- Functional polyamine metabolic enzymes and pathways encoded by the virosphere.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Streptococcus pyogenes Φ1207.3 Is a Temperate Bacteriophage Carrying the Macrolide Resistance Gene PairMicrobiology spectrum · 2023Article
- An Overview of Macrolide Resistance in Streptococci: Prevalence, Mobile Elements and Dynamics.Microorganisms · 2022Review
- How Streptococcus suis escapes antibiotic treatments.Veterinary research · 2022Review
- Mobile genetic elements used by competing coral microbial populations increase genomic plasticity.The ISME journal · 2022Article
- Characterization of integrative and conjugative elements carrying antibiotic resistance genes ofFrontiers in microbiology · 2022Article
- Predicted transmembrane proteins with homology to Mef(A) are not responsible for complementing mef(A) deletion in the mef(A)-msr(D) macrolide efflux system in Streptococcus pneumoniae.BMC research notes · 2021Article
- Fitness benefits to bacteria of carrying prophages and prophage-encoded antibiotic-resistance genes peak in different environments.Evolution; international journal of organic evolution · 2021Article
- New Insights onFrontiers in microbiology · 2021Article
- Characterization of Clinical and CarrierViruses · 2020Article
- Characterization and spontaneous induction of urinary tractThe Journal of general virology · 2020Article
- Characterization of Bacteriophage Peptides of PathogenicFrontiers in microbiology · 2020Article
- The Bacteriophages ofMicrobiology spectrum · 2019Review
- Characterization of a Linezolid- and Vancomycin-ResistantFrontiers in microbiology · 2019Article
- Characterization of Streptococcus pluranimalium from a cattle with mastitis by whole genome sequencing and functional validation.BMC microbiology · 2018Article
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Phim46.1, the recognized representative of the most common variant of mobile, prophage-associated genetic elements carrying resistance genes mef(A) (which confers efflux-mediated erythromycin resistance) and tet(O) (which confers tetracycline resistance) in Streptococcus pyogenes, was fully characterized. Sequencing of the Phim46.1 genome (55,172 bp) demonstrated a modular organization typical of tailed bacteriophages. Electron microscopic analysis of mitomycin-induced Phim46.1 revealed phage particles with the distinctive icosahedral head and tail morphology of the Siphoviridae family. The chromosome integration site was within a 23S rRNA uracil methyltransferase gene. BLASTP analysis revealed that the proteins of Phim46.1 had high levels of amino acid sequence similarity to the amino acid sequences of proteins from other prophages, especially Phi10394.4 of S. pyogenes and lambdaSa04 of S. agalactiae. Phage DNA was present in the host cell both as a prophage and as free circular DNA. The lysogeny module appears to have been split due to the insertion of a segment containing tet(O) (from integrated conjugative element 2096-RD.2) and mef(A) (from a Tn1207.1-like transposon) into the unintegrated phage DNA. The phage attachment sequence lies in the region between tet(O) and mef(A) in the unintegrated form. Thus, whereas in this form tet(O) is approximately 5.5 kb upstream of mef(A), in the integrated form, tet(O), which lies close to the right end of the prophage, is approximately 46.3 kb downstream of mef(A), which lies close to the left end of the prophage.
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