ArticleNucleic acids research2010
Transposition of the human Hsmar1 transposon: rate-limiting steps and the importance of the flanking TA dinucleotide in second strand cleavage.
Article in Nucleic acids research, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
32 citing papers in PubMed, 45 citations in OpenAlex.
- Article
- Jump ahead with a twist: DNA acrobatics drive transposition forward.Current opinion in structural biology · 2019Review
- Targeted DNA transposition in vitro using a dCas9-transposase fusion protein.Nucleic acids research · 2019Article
- Structural insights into the mechanism of double strand break formation by Hermes, a hAT family eukaryotic DNA transposase.Nucleic acids research · 2018Article
- Transposase subunit architecture and its relationship to genome size and the rate of transposition in prokaryotes and eukaryotes.Nucleic acids research · 2018Article
- A single active site in the mariner transposase cleaves DNA strands of opposite polarity.Nucleic acids research · 2017Article
- SVA retrotransposons as potential modulators of neuropeptide gene expression.Neuropeptides · 2017Review
- DNA Transposition at Work.Chemical reviews · 2016Review
- Sleeping Beauty transposase structure allows rational design of hyperactive variants for genetic engineering.Nature communications · 2016Article
- Biochemical Characterization of Kat1: a Domesticated hAT-Transposase that Induces DNA Hairpin Formation and MAT-Switching.Scientific reports · 2016Article
- Evolutionary history of the Azteca-like mariner transposons and their host ants.Die Naturwissenschaften · 2015Article
- Mechanisms of DNA Transposition.Microbiology spectrum · 2015Review
- Structural role of the flanking DNA in mariner transposon excision.Nucleic acids research · 2015Article
- Crosstalk between transposase subunits during cleavage of the mariner transposon.Nucleic acids research · 2014Article
- Suicidal autointegration of sleeping beauty and piggyBac transposons in eukaryotic cells.PLoS genetics · 2014Article
- Biochemical characterization and comparison of two closely related active mariner transposases.Biochemistry · 2014Article
- Hyperactive mariner transposons are created by mutations that disrupt allosterism and increase the rate of transposon end synapsis.Nucleic acids research · 2014Article
- Target capture during Mos1 transposition.The Journal of biological chemistry · 2014Article
- One to rule them all: A highly conserved motif in mariner transposase controls multiple steps of transposition.Mobile genetic elements · 2014Article
- Article
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Authors and funding
2 authors at 2 institutions in 1 country.
Funding
Abstract
Hsmar1 is a member of the mariner family of DNA transposons. Although widespread in nature, their molecular mechanism remains obscure. Many other cut-and-paste elements use a hairpin intermediate to cleave the two strands of DNA at each transposon end. However, this intermediate is absent in mariner, suggesting that these elements use a fundamentally different mechanism for second-strand cleavage. We have taken advantage of the faithful and efficient in vitro reaction provided by Hsmar1 to characterize the products and intermediates of transposition. We report different factors that particularly affect the reaction, which are the reaction pH and the transposase concentration. Kinetic analysis revealed that first-strand nicking and integration are rapid. The rate of the reaction is limited in part by the divalent metal ion-dependent assembly of a complex between transposase and the transposon end(s) prior to the first catalytic step. Second-strand cleavage is the rate-limiting catalytic step of the reaction. We discuss our data in light of a model for the two metal ion catalytic mechanism and propose that mariner excision involves a significant conformational change between first- and second-strand cleavage at each transposon end. Furthermore, this conformational change requires specific contacts between transposase and the flanking TA dinucleotide.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.