ArticleNucleic acids research2010
Telomeric circles are abundant in the stn1-M1 mutant that maintains its telomeres through recombination.
Article in Nucleic acids research, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers, 1 of them a synthesis that pooled it.
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Who cites it
14 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Characterization, biogenesis model, and current bioinformatics of human extrachromosomal circular DNA.Frontiers in genetics · 2024Pooled it
- Unveiling the mysteries of extrachromosomal circular DNA: from generation to clinical relevance in human cancers and health.Molecular cancer · 2024Review
- Extrachromosomal DNA: Molecular perspectives in aging and neurodegenerative diseases.Intractable & rare diseases research · 2024Article
- Imaging extrachromosomal DNA (ecDNA) in cancer.Histochemistry and cell biology · 2024Review
- Gene amplifications and extrachromosomal circular DNAs: function and biogenesis.Molecular biology reports · 2023Review
- Telomerase, the recombination machinery and Rap1 play redundant roles in yeast telomere protection.Current genetics · 2021Article
- Current understanding of extrachromosomal circular DNA in cancer pathogenesis and therapeutic resistance.Journal of hematology & oncology · 2020Review
- RAD51 and RTEL1 compensate telomere loss in the absence of telomerase.Nucleic acids research · 2018Article
- Review
- "Poisoning" yeast telomeres distinguishes between redundant telomere capping pathways.Chromosoma · 2012Article
- Maintenance of very long telomeres by recombination in the Kluyveromyces lactis stn1-M1 mutant involves extreme telomeric turnover, telomeric circles, and concerted telomeric amplification.Molecular and cellular biology · 2012Article
- Caenorhabditis elegans POT-2 telomere protein represses a mode of alternative lengthening of telomeres with normal telomere lengths.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- Long telomeres produced by telomerase-resistant recombination are established from a single source and are subject to extreme sequence scrambling.PLoS genetics · 2012Article
- Recombination can either help maintain very short telomeres or generate longer telomeres in yeast cells with weak telomerase activity.Eukaryotic cell · 2011Article
Corrections and comments
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Authors and funding
5 authors.
Funding
Abstract
Some human cancers maintain their telomeres using the alternative lengthening of telomeres (ALT) mechanism; a process thought to involve recombination. Different types of recombinational telomere elongation pathways have been identified in yeasts. In senescing yeast telomerase deletion (ter1-Delta) mutants with very short telomeres, it has been hypothesized that copying a tiny telomeric circle (t-circle) by a rolling circle mechanism is the key event in telomere elongation. In other cases more closely resembling ALT cells, such as the stn1-M1 mutant of Kluyveromyces lactis, the telomeres appear to be continuously unstable and routinely reach very large sizes. By employing two-dimensional gel electrophoresis and electron microscopy, we show that stn1-M1 cells contain abundant double stranded t-circles ranging from approximately 100 to 30,000 bp in size. We also observed small single-stranded t-circles, specifically composed of the G-rich telomeric strand and tailed circles resembling rolling circle replication intermediates. The t-circles most likely arose from recombination events that also resulted in telomere truncations. The findings strengthen the possibility that t-circles contribute to telomere maintenance in stn1-M1 and ALT cells.
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Registered trials
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