ArticleJournal of controlled release : official journal of the Controlled Release Society2010
PEGylation of bacterial cocaine esterase for protection against protease digestion and immunogenicity.
Article in Journal of controlled release : official journal of the Controlled Release Society, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
18 citing papers in PubMed, 36 citations in OpenAlex.
- Tracer Diffusivity in Amphiphilic Polymer Model Co-Networks.Macromolecules · 2026Article
- Optimizing Pharmacological and Immunological Properties of Therapeutic Proteins Through PEGylation: Investigating Key Parameters and Their Impact.Drug design, development and therapy · 2024Review
- Research progress on the PEGylation of therapeutic proteins and peptides (TPPs).Frontiers in pharmacology · 2024Review
- Expression Profiling Reveals the Possible Involvement of the Ubiquitin-Proteasome Pathway in Abiotic Stress Regulation inInternational journal of molecular sciences · 2023Article
- Intra-articular kinetics of a cartilage targeting cationic PEGylated protein for applications in drug delivery.Osteoarthritis and cartilage · 2023Article
- Enhanced control of bioactivity of tissue plasminogen activator (tPA) through domain-directed enzymatic oxidation of terminal galactose.BioImpacts : BI · 2022Article
- PEGylation of a High-Affinity Anti-(+)Methamphetamine Single Chain Antibody Fragment Extends Functional Half-Life by Reducing Clearance.Pharmaceutical research · 2016Article
- Shaping bio-inspired nanotechnologies to target thrombosis for dual optical-magnetic resonance imaging.Journal of materials chemistry. B · 2015Article
- Infusion of imaging and therapeutic molecules into the plant virus-based carrier cowpea mosaic virus: cargo-loading and delivery.Journal of controlled release : official journal of the Controlled Release Society · 2013Article
- Engineering Gd-loaded nanoparticles to enhance MRI sensitivity via T(1) shortening.Nanotechnology · 2013Review
- Design rules for nanomedical engineering: from physical virology to the applications of virus-based materials in medicine.Journal of biological physics · 2013Review
- Cell permeable cocaine esterases constructed by chemical conjugation and genetic recombination.Molecular pharmaceutics · 2012Article
- Bacterial cocaine esterase: a protein-based therapy for cocaine overdose and addiction.Future medicinal chemistry · 2012Review
- Pharmacokinetic strategies for treatment of drug overdose and addiction.Future medicinal chemistry · 2012Review
- The fate of bacterial cocaine esterase (CocE): an in vivo study of CocE-mediated cocaine hydrolysis, CocE pharmacokinetics, and CocE elimination.The Journal of pharmacology and experimental therapeutics · 2012Article
- Subunit stabilization and polyethylene glycolation of cocaine esterase improves in vivo residence time.Molecular pharmacology · 2011Article
- Computational design of protein-ligand interfaces: potential in therapeutic development.Trends in biotechnology · 2011Review
- Enzyme-therapy approaches for the treatment of drug overdose and addiction.Future medicinal chemistry · 2011Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors at 1 institution in 1 country.
Funding
Abstract
Enhancing cocaine metabolism by administration of cocaine esterase (CocE) has been considered as a promising treatment strategy for cocaine overdose and addiction, as CocE is the most efficient native enzyme yet identified for metabolizing the naturally occurring cocaine. A major obstacle to the clinical application of CocE, however, lies in its thermo-instability, rapid degradation by circulating proteases, and potential immunogenicity. PEGylation, namely by modifying a protein or peptide compound via attachment of polyethylene glycol (PEG) chains, has been proven to overcome such problems and was therefore exploited in this CocE investigation. The PEG-CocE conjugates prepared in this study showed a purity of greater than 93.5%. Attachment of PEG to CocE apparently inhibited the binding of anti-CocE antibodies to the conjugate, as demonstrated by the enzyme-linked immunosorbent assay (ELISA) assay. In addition, PEGylation yielded protection to CocE against thermal degradation and protease digestion. Furthermore, preliminary in vivo results suggested that, similarly to native CocE, the PEG-CocE conjugates were able to protect animals from cocaine-induced toxic effects. Overall, this study provides evidence that the PEGylation may serve as a tool to prolong CocE functionality in the circulation and reduce its potential immunogenicity.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.