ArticleJournal of proteome research2009
Liquid chromatography-tandem and MALDI imaging mass spectrometry analyses of RCL2/CS100-fixed, paraffin-embedded tissues: proteomics evaluation of an alternate fixative for biomarker discovery.
Article in Journal of proteome research, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
18 citing papers in PubMed, 51 citations in OpenAlex.
- Light sheet fluorescence microscopy guided MALDI-imaging mass spectrometry of cleared tissue samples.Scientific reports · 2020Article
- The role of the pathology department in the preanalytical phase of molecular analyses.Cancer management and research · 2018Article
- Future technology insight: mass spectrometry imaging as a tool in drug research and development.British journal of pharmacology · 2015Review
- A review of room temperature storage of biospecimen tissue and nucleic acids for anatomic pathology laboratories and biorepositories.Clinical biochemistry · 2014Review
- Advances in Proteomic Technologies and Its Contribution to the Field of Cancer.Advances in medicine · 2014Review
- Mass spectrometry imaging with high resolution in mass and space.Histochemistry and cell biology · 2013Review
- Current status and future perspectives of mass spectrometry imaging.International journal of molecular sciences · 2013Review
- MALDI imaging mass spectrometry for direct tissue analysis: technological advancements and recent applications.Histochemistry and cell biology · 2011Review
- From whole-body sections down to cellular level, multiscale imaging of phospholipids by MALDI mass spectrometry.Molecular & cellular proteomics : MCP · 2011Article
- Targeted therapies in cancer - challenges and chances offered by newly developed techniques for protein analysis in clinical tissues.Journal of Cancer · 2010Article
- Mass-spectrometry based oxidative lipidomics and lipid imaging: applications in traumatic brain injury.Journal of neurochemistry · 2010Review
- Fixation of brain tumor biopsy specimens with RCL2 results in well-preserved histomorphology, immunohistochemistry and nucleic acids.Brain pathology (Zurich, Switzerland) · 2010Article
- MALDI imaging and profiling MS of higher mass proteins from tissue.Journal of the American Society for Mass Spectrometry · 2010Article
- Molecular mass spectrometry imaging in biomedical and life science research.Histochemistry and cell biology · 2010Review
- Mass spectrometric imaging for biomedical tissue analysis.Chemical reviews · 2010Review
- Histo-proteomic profiling of formalin-fixed, paraffin-embedded tissue.Expert review of proteomics · 2010Review
- Matrix-assisted laser desorption/ionization imaging mass spectrometry.International journal of molecular sciences · 2010Review
- KINFix--A formalin-free non-commercial fixative optimized for histological, immunohistochemical and molecular analyses of neurosurgical tissue specimens.Clinical neuropathologyArticle
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
Human tissues are an important source of biological material for the discovery of novel biomarkers. Fresh-frozen tissue could represent an ideal supply of archival material for molecular investigations. However, immediate flash freezing is usually not possible, especially for rare or valuable tissue samples such as biopsies. Here, we investigated the compatibility of RCL2/CS100, a non-cross-linking, nontoxic, and nonvolatile organic fixative, with shotgun proteomic analyses. Several protein extraction protocols compatible with mass spectrometry were investigated from RCL2/CS100-fixed and fresh-frozen colonic mucosa, breast, and prostate tissues. The peptides and proteins identified from RCL2/CS100 tissue were then comprehensively compared with those identified from matched fresh-frozen tissues using a bottom-up strategy based on nano-reversed phase liquid chromatography coupled with tandem mass spectrometry (nanoRPLC-MS/MS). Results showed that similar peptides could be identified in both archival conditions and the proteome coverage was not obviously compromised by the RCL2/CS100 fixation process. NanoRPLC-MS/MS of laser capture microdissected RCL2/CS100-fixed tissues gave the same amount of biological information as that recovered from whole RCL2/CS100-fixed or frozen tissues. We next performed MALDI tissue profiling and imaging mass spectrometry and observed a high level of agreement in protein expression as well as excellent agreement between the images obtained from RCL2/CS100-fixed and fresh-frozen tissue samples. These results suggest that RCL2/CS100-fixed tissues are suitable for shotgun proteomic analyses and tissue imaging. More importantly, this alternate fixative opens the door to the analysis of small, valuable, and rare target lesions that are usually inaccessible to complementary biomarker-driven genomic and proteomic research.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.