ArticlePediatric surgery international2010
Transglutaminases, involucrin, and loricrin as markers of epidermal differentiation in skin substitutes derived from human sweat gland cells.
Article in Pediatric surgery international, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 42 citations in OpenAlex.
- Decoding Sebaceous Gland Biology Through Experimental Models: Progress, Limitations, and Future Directions.Cells · 2026Review
- Hydrodynamically generated multilayer skin spheroids enable in vitro screening of biologically active ingredients and toxicity tests.Science advances · 2025Article
- Topical administration of the secretome derived from human amniotic epithelial cells ameliorates psoriasis-like skin lesions in mice.Stem cell research & therapy · 2022Article
- Autoantigen Discovery in the Hair Loss Disorder, Alopecia Areata: Implication of Post-Translational Modifications.Frontiers in immunology · 2022Review
- Role of Two-Pore Channels in Embryonic Development and Cellular Differentiation.Cold Spring Harbor perspectives in biology · 2020Review
- Establishment of a complex skin structure via layered co-culture of keratinocytes and fibroblasts derived from induced pluripotent stem cells.Stem cell research & therapy · 2018Article
- Localization of serine racemase and its role in the skin.The Journal of investigative dermatology · 2014Article
- Enhanced skin wound healing by a sustained release of growth factors contained in platelet-rich plasma.Experimental & molecular medicine · 2011Article
- Skingineering II: transplantation of large-scale laboratory-grown skin analogues in a new pig model.Pediatric surgery international · 2011Article
- Skingineering I: engineering porcine dermo-epidermal skin analogues for autologous transplantation in a large animal model.Pediatric surgery international · 2011Article
- Determining the origin of cells in tissue engineered skin substitutes: a pilot study employing in situ hybridization.Pediatric surgery international · 2011Article
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
background/purposeIn a multi-project research line, we are currently testing whether a morphologically and functionally near normal epidermis can be cultured from human sweat gland (SG) cells and be used as a skin substitute. The present study focuses on the stratum corneum of the epidermis that assumes a vital barrier function for the skin. The main process in the formation of the cornified cell envelope in human epidermis, i.e. crosslinking of proteins and lipids, is catalyzed by several transglutaminases (TG). Therefore, we compared the expression patterns of various TG and their substrates in SG-derived versus keratinocyte-derived epidermal substitutes.
methodsSweat gland cells, keratinocytes, and fibroblasts were isolated from human skin samples and cultivated separately to generate epidermal substitutes. These were transplanted onto the back of athymic rats. After 2 weeks, the transplants were excised and analyzed histologically as well as by indirect immunofluorescence. We looked at the expression of TG1, 3, 5, and their substrates involucrin and loricrin (=markers of epidermal differentiation) in SG-derived and keratinocyte-derived skin substitutes as well as in normal skin.
resultsThe SG cell-derived epidermis was near normal anatomically, formed a cornified cell envelope and demonstrated TG1, 3, and 5 as well as involucrin and loricrin expression patterns similar to those found in keratinocyte-derived epidermis and normal control skin.
conclusionThese findings support the thesis that SG cells have the potential to form a near normal stratified epidermal analog that might be used as a skin substitute. The expression of TG1 and 3, not normally expressed in human SG, suggests the presence of re-programmed SG cells and/or stem cells capable of both de novo generating and maintaining an epidermis.
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