ArticleJournal of structural and functional genomics2009
Production of membrane proteins for NMR studies using the condensed single protein (cSPP) production system.
Article in Journal of structural and functional genomics, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 27 citations in OpenAlex.
- Suppression of the toxicity of Bac7 (1-35), a bovine peptide antibiotic, and its production in E. coli.AMB Express · 2016Article
- The quiet renaissance of protein nuclear magnetic resonance.Biochemistry · 2013Review
- Membrane proteins, magic-angle spinning, and in-cell NMR.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- Regulation of growth and death in Escherichia coli by toxin-antitoxin systems.Nature reviews. Microbiology · 2011Review
- In situ structural characterization of a recombinant protein in native Escherichia coli membranes with solid-state magic-angle-spinning NMR.Journal of the American Chemical Society · 2011Article
- The use of the condensed single protein production system for isotope-labeled outer membrane proteins, OmpA and OmpX in E. coli.Molecular biotechnology · 2011Article
- Suppression of phospholipid biosynthesis by cerulenin in the condensed Single-Protein-Production (cSPP) system.Journal of biomolecular NMR · 2011Article
- Secretion of human superoxide dismutase in Escherichia coli using the condensed single-protein-production system.Protein science : a publication of the Protein Society · 2010Article
- Condensed E. coli cultures for highly efficient production of proteins containing unnatural amino acids.Bioorganic & medicinal chemistry letters · 2010Article
- Use of amino acids as inducers for high-level protein expression in the single-protein production system.Applied and environmental microbiology · 2010Article
- Advances in protein NMR provided by the NIGMS Protein Structure Initiative: impact on drug discovery.Current opinion in drug discovery & development · 2010Review
- The E. coli single protein production system for production and structural analysis of membrane proteins.Journal of structural and functional genomics · 2010Article
- A microscale protein NMR sample screening pipeline.Journal of biomolecular NMR · 2010Article
Corrections and comments
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Authors and funding
10 authors at 3 institutions in 1 country.
Funding
Abstract
In the Single Protein Production (SPP) method, all E. coli cellular mRNAs are eliminated by the induction of MazF, an ACA-specific mRNA interferase. When an mRNA for a membrane protein, engineered to have no ACA sequences without altering its amino acid sequence, is induced in the MazF-induced cells, E. coli is converted into a bioreactor producing only the targeted membrane protein. Here we demonstrate that three prokaryotic inner membrane proteins, two prokaryotic outer membrane proteins, and one human virus membrane protein can be produced at very high levels, and assembled in appropriate membrane fractions. The condensed SPP (cSPP) system was used to selectively produce isotope-enriched membrane proteins for NMR studies in up to 150-fold condensed culture without affecting protein yields, providing more than 99% cost saving for isotopes. As a novel application of the cSPP system for studies of membrane proteins prior to purification we also demonstrate, for the first time, fast detergent screening by microcoil NMR and well-resolved NMR spectra of several targeted integral membrane proteins obtained without purification.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.