ArticlePloS one2009
Tyrosine, cysteine, and S-adenosyl methionine stimulate in vitro [FeFe] hydrogenase activation.
Article in PloS one, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed, 72 citations in OpenAlex.
- Ultrasound-assisted enhancement in biohydrogen production from food waste hydrolysate: a metabolic flux analysis.Bioprocess and biosystems engineering · 2026Article
- In vitro maturation of fully active [FeFe]-hydrogenase in a defined system including the iron carrier NfuA.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- HydE Catalytic Mechanism Is Powered by a Radical Relay with Redox-Active Fe(I)-Containing Intermediates.Journal of the American Chemical Society · 2025Article
- Genome-Scale Mining of Acetogens of the GenusMicrobiology spectrum · 2022Article
- [FeFe]-Hydrogenase: Defined Lysate-Free Maturation Reveals a Key Role for Lipoyl-H-Protein in DTMA Ligand Biosynthesis.Angewandte Chemie (International ed. in English) · 2022Article
- In Vitro Biosynthesis of the [Fe]-Hydrogenase Cofactor Verifies the Proposed Biosynthetic Precursors.Angewandte Chemie (International ed. in English) · 2022Article
- Radical SAM Enzymes and Metallocofactor Assembly: A Structural Point of View.ACS bio & med chem Au · 2022Review
- Capture of carbon dioxide and hydrogen by engineered Escherichia coli: hydrogen-dependent COApplied microbiology and biotechnology · 2021Article
- Biosynthesis of the [FeFe] Hydrogenase H Cluster: A Central Role for the Radical SAM Enzyme HydG.Inorganic chemistry · 2016Article
- Functional properties of flagellin as a stimulator of innate immunity.Scientific reports · 2016Article
- Atypical effect of temperature tuning on the insertion of the catalytic iron-sulfur center in a recombinant [FeFe]-hydrogenase.Protein science : a publication of the Protein Society · 2015Article
- Cysteine as a ligand platform in the biosynthesis of the FeFe hydrogenase H cluster.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- EPR Spectroscopic Studies of [FeFe]-Hydrogenase Maturation.Catalysis letters · 2015Article
- [FeFe]-hydrogenase maturation: insights into the role HydE plays in dithiomethylamine biosynthesis.Biochemistry · 2015Article
- Chemical lysis of cyanobacteria.Journal of biological engineering · 2015Article
- Reversible H atom abstraction catalyzed by the radical S-adenosylmethionine enzyme HydG.Journal of the American Chemical Society · 2014Article
- H-cluster assembly during maturation of the [FeFe]-hydrogenase.Journal of biological inorganic chemistry : JBIC : a publication of the Society of Biological Inorganic Chemistry · 2014Review
- Radical S-adenosylmethionine enzymes.Chemical reviews · 2014Review
- Article
- Cell-free production of trimeric influenza hemagglutinin head domain proteins as vaccine antigens.Biotechnology and bioengineering · 2012Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
background[FeFe] hydrogenases are metalloenzymes involved in the anaerobic metabolism of H(2). These proteins are distinguished by an active site cofactor known as the H-cluster. This unique [6Fe-6S] complex contains multiple non-protein moieties and requires several maturation enzymes for its assembly. The pathways and biochemical precursors for H-cluster biosynthesis have yet to be elucidated. PRINCIPAL
findingsWe report an in vitro maturation system in which, for the first time, chemical additives enhance [FeFe] hydrogenase activation, thus signifying in situ H-cluster biosynthesis. The maturation system is comprised of purified hydrogenase apoprotein; a dialyzed Escherichia coli cell lysate containing heterologous HydE, HydF, and HydG maturases; and exogenous small molecules. Following anaerobic incubation of the Chlamydomonas reinhardtii HydA1 apohydrogenase with S-adenosyl methionine (SAM), cysteine, tyrosine, iron, sulfide, and the non-purified maturases, hydrogenase activity increased 5-fold relative to incubations without the exogenous substrates. No conditions were identified in which addition of guanosine triphosphate (GTP) improved hydrogenase maturation. SIGNIFICANCE: The in vitro system allows for direct investigation of [FeFe] hydrogenase activation. This work also provides a foundation for studying the biosynthetic mechanisms of H-cluster biosynthesis using solely purified enzymes and chemical additives.
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