ArticleNature methods2009
'Edgetic' perturbation of a C. elegans BCL2 ortholog.
Article in Nature methods, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 38 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
38 citing papers in PubMed, 67 citations in OpenAlex.
- Article
- The Extent of Edgetic Perturbations in the Human Interactome Caused by Population-Specific Mutations.Biomolecules · 2023Article
- Discovery of Native Protein Complexes by Liquid Chromatography Followed by Quantitative Mass Spectrometry.Advances in experimental medicine and biology · 2021Article
- Domain-mediated interactions for protein subfamily identification.Scientific reports · 2020Article
- bZIP edgetic mutations: at the frontier of plant metabolism, development and stress trade-off.Journal of experimental botany · 2019Article
- Dynein links engulfment and execution of apoptosis via CED-4/Apaf1 in C. elegans.Cell death & disease · 2018Article
- Base-resolution stratification of cancer mutations using functional variomics.Nature protocols · 2017Article
- Identification and characterization of a novel Sso7d scaffold-based binder against Notch1.Scientific reports · 2017Article
- Constraints and consequences of the emergence of amino acid repeats in eukaryotic proteins.Nature structural & molecular biology · 2017Article
- Large-scale SNP screenings identify markers linked with GCRV resistant traits through transcriptomes of individuals and cell lines in Ctenopharyngodon idella.Scientific reports · 2017Article
- A Split-Ubiquitin Based Strategy Selecting for Protein Complex-Interfering Mutations.G3 (Bethesda, Md.) · 2016Article
- NetDecoder: a network biology platform that decodes context-specific biological networks and gene activities.Nucleic acids research · 2016Article
- Identification by Random Mutagenesis of Functional Domains in KREPB5 That Differentially Affect RNA Editing between Life Cycle Stages of Trypanosoma brucei.Molecular and cellular biology · 2015Article
- Next-Generation Sequencing for Binary Protein-Protein Interactions.Frontiers in genetics · 2015Article
- Cell Death in C. elegans Development.Current topics in developmental biology · 2015Review
- The yeast two-hybrid assay: still finding connections after 25 years.Nature methods · 2014Article
- Systematic exploration of ubiquitin sequence, E1 activation efficiency, and experimental fitness in yeast.Journal of molecular biology · 2014Article
- Mutations that disrupt PHOXB interaction with the neuronal calcium sensor HPCAL1 impede cellular differentiation in neuroblastoma.Oncogene · 2014Article
- High-resolution network biology: connecting sequence with function.Nature reviews. Genetics · 2013Review
- Edgotype: a fundamental link between genotype and phenotype.Current opinion in genetics & development · 2013Review
Corrections and comments
- Commented on by
- Erratum issued
Authors and funding
15 authors at 4 institutions in 3 countries.
Funding
Abstract
Genes and gene products do not function in isolation but within highly interconnected 'interactome' networks, modeled as graphs of nodes and edges representing macromolecules and interactions between them, respectively. We propose to investigate genotype-phenotype associations by methodical use of alleles that lack single interactions, while retaining all others, in contrast to genetic approaches designed to eliminate gene products completely. We describe an integrated strategy based on the reverse yeast two-hybrid system to isolate and characterize such edge-specific, or 'edgetic', alleles. We established a proof of concept with CED-9, a Caenorhabditis elegans BCL2 ortholog. Using ced-9 edgetic alleles, we uncovered a new potential functional link between apoptosis and a centrosomal protein. This approach is amenable to higher throughput and is particularly applicable to interactome network analysis in organisms for which transgenesis is straightforward.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.