ArticleAmerican journal of physiology. Heart and circulatory physiology2010
Role of local production of endothelium-derived nitric oxide on cGMP signaling and S-nitrosylation.
Article in American journal of physiology. Heart and circulatory physiology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 36 citations in OpenAlex.
- Editorial: Integrative endothelial signaling mechanisms in resistance arteries.Frontiers in physiology · 2026Article
- Vasodilation and Blood Pressure-Lowering Effect of 3-Demethyl-2-Geranyl-4-Prenylbellidifoline, a Xanthone Obtained fromPlants (Basel, Switzerland) · 2024Article
- Redox Switches Controlling Nitric Oxide Signaling in the Resistance Vasculature and Implications for Blood Pressure Regulation: Mid-Career Award for Research Excellence 2020.Hypertension (Dallas, Tex. : 1979) · 2021Review
- α-galactosidase A deficiency promotes von Willebrand factor secretion in models of Fabry disease.Kidney international · 2019Article
- Can endothelial hemoglobin-α regulate nitric oxide vasodilatory signaling?American journal of physiology. Heart and circulatory physiology · 2017Article
- Regulation of gap junction channels and hemichannels by phosphorylation and redox changes: a revision.BMC cell biology · 2016Review
- Endothelial nitric oxide synthase in the microcirculation.Cellular and molecular life sciences : CMLS · 2015Review
- Stromal cell-derived factor 2 is critical for Hsp90-dependent eNOS activation.Science signaling · 2015Article
- Hemoglobin α in the blood vessel wall.Free radical biology & medicine · 2014Review
- S-nitrosation of mitochondrial connexin 43 regulates mitochondrial function.Basic research in cardiology · 2014Article
- Recent insights in the paracrine modulation of cardiomyocyte contractility by cardiac endothelial cells.BioMed research international · 2014Review
- Post-translational regulation of endothelial nitric oxide synthase in vascular endothelium.Frontiers in physiology · 2013Review
- Specificity in S-nitrosylation: a short-range mechanism for NO signaling?Antioxidants & redox signaling · 2013Review
- Myosin light chain kinase signaling in endothelial barrier dysfunction.Medicinal research reviews · 2013Review
- Insights into the arginine paradox: evidence against the importance of subcellular location of arginase and eNOS.American journal of physiology. Heart and circulatory physiology · 2013Article
- Ventilatory responses during and following exposure to a hypoxic challenge in conscious mice deficient or null in S-nitrosoglutathione reductase.Respiratory physiology & neurobiology · 2013Article
- Interaction between nitric oxide signaling and gap junctions: effects on vascular function.Biochimica et biophysica acta · 2012Review
- Nitric oxide reduces NADPH oxidase 5 (Nox5) activity by reversible S-nitrosylation.Free radical biology & medicine · 2012Article
- Molecular characterization of myosin phosphatase in endothelium.Journal of cellular physiology · 2012Article
- Exogenous, but not Endogenous Nitric Oxide Inhibits Adhesion Molecule Expression in Human Endothelial Cells.Frontiers in physiology · 2012Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
Nitric oxide (NO), synthesized by endothelial nitric oxide synthase (eNOS), exerts control over vascular function via two distinct mechanisms, the activation of soluble guanylate cyclase (sGC)/cGMP-dependent signaling or through S-nitrosylation of proteins with reactive thiols (S-nitrosylation). Previous studies in cultured endothelial cells revealed that eNOS targeted to the plasma membrane (PM) releases greater amounts of NO compared with Golgi tethered eNOS. However, the significance of eNOS localization to sGC-dependent or -independent signaling is not known. Here we show that PM-targeted eNOS, when expressed in human aortic endothelial cells (HAEC) and isolated blood vessels, increases sGC/cGMP signaling to a greater extent than Golgi-localized eNOS. The ability of local NO production to influence sGC-independent mechanisms was also tested by monitoring the secretion of Von Willebrand factor (vWF), which is tonically inhibited by the S-nitrosylation of N-ethylmaleimide sensitive factor (NSF). In eNOS "knockdown" HAECs, vWF secretion was attenuated to a greater degree by PM eNOS compared with a Golgi-restricted eNOS. Moreover, the PM-targeted eNOS induced greater S-nitrosylation of NSF vs. Golgi eNOS. To distinguish between the amount of NO generated and the intracellular location of synthesis, we expressed Golgi and PM-targeted calcium-insensitive forms of eNOS in HAEC. These constructs, which generate equal amounts of NO regardless of location, produced equivalent increases in cGMP in bioassays and equal inhibition of vWF secretion. We conclude that the greater functional effects of PM eNOS are due to the increased amount of NO produced rather than effects derived from the local synthesis of NO.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.