ArticleProceedings of the National Academy of Sciences of the United States of America2009
Exon sequences at the splice junctions affect splicing fidelity and alternative splicing.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 31 citations in OpenAlex.
- Synonymous and non-synonymous variants at splice junctions can disrupt splicing and are frequently linked to disease associated loss of function genes.BMC genomics · 2025Article
- Splicing factor Prp18p promotes genome-wide fidelity of consensus 3'-splice sites.Nucleic acids research · 2023Article
- Predominant Liver Cystic Disease in a New Heterozygotic PKHD1 Variant: A Case Report.The American journal of case reports · 2023Article
- Adenosine Deaminase 2 Deficiency Caused by Biallele Variants Including Splicing Variant: The First Case in Korea.Journal of rheumatic diseases · 2022Article
- Molecular Analysis ofFrontiers in genetics · 2020Article
- RNA splicing process analysis for identifying antisense oligonucleotide inhibitors with padlock probe-based isothermal amplification.Chemical science · 2017Article
- The Fission Yeast Pre-mRNA-processing Factor 18 (prp18+) Has Intron-specific Splicing Functions with Links to G1-S Cell Cycle Progression.The Journal of biological chemistry · 2016Article
- RNA-Binding Proteins: Splicing Factors and Disease.Biomolecules · 2015Review
- Cwc21p promotes the second step conformation of the spliceosome and modulates 3' splice site selection.Nucleic acids research · 2015Article
- Article
- Widespread use of non-productive alternative splice sites in Saccharomyces cerevisiae.PLoS genetics · 2014Article
- CEF1/CDC5 alleles modulate transitions between catalytic conformations of the spliceosome.RNA (New York, N.Y.) · 2012Article
- Determinants of Nam8-dependent splicing of meiotic pre-mRNAs.Nucleic acids research · 2011Article
- Factors affecting splicing strength of yeast genes.Comparative and functional genomics · 2011Article
- Allele-specific recognition of the 3' splice site of INS intron 1.Human genetics · 2010Article
- Mechanisms and regulation of spliceosome-mediated pre-mRNA splicing in Saccharomyces cerevisiae.Wiley interdisciplinary reviews. RNAReview
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Identification of splice sites is essential for the expression of most eukaryotic genes, allowing accurate splicing of pre-mRNAs. The splice sites are recognized by the splicing machinery based on sequences within the pre-mRNA. Here, we show that the exon sequences at the splice junctions play a significant, previously unrecognized role in the selection of 3' splice sites during the second step of splicing. The influence of the exon sequences was enhanced by the Prp18 mutant Prp18DeltaCR, and the strength of an exon sequence in Prp18DeltaCR splicing predicted its effect in wild-type splicing. Analysis of the kinetics of splicing in vitro demonstrated that 3' splice sites were chosen competitively during the second step, likely at the same time as exon ligation. In wild-type yeast, splice site selection for two genes studied was altered by point mutations in their exon bases, affecting splicing fidelity and alternative splicing. Finally, we note that the degeneracy of the genetic code allows competing 3' splice sites to be eliminated from coding regions, and we suggest that the evolution of the splicing signals and the genetic code are connected.
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