ArticleNucleic acids research2010
Drosophila mini-white model system: new insights into positive position effects and the role of transcriptional terminators and gypsy insulator in transgene shielding.
Article in Nucleic acids research, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 33 citations in OpenAlex.
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- A PRE loop at the dac locus acts as a topological chromatin structure that restricts and specifies enhancer-promoter communication.Nature structural & molecular biology · 2024Article
- Orange maker: a CRISPR/Cas9-mediated genome editing and screening project to generate orange-eyed DarkJedi GAL4 lines by undergraduate students.Scientific reports · 2024Article
- CRISPR/Cas9-mediated efficient white genome editing in the black soldier fly Hermetia illucens.Molecular genetics and genomics : MGG · 2024Article
- Corazonin Neurons Contribute to Dimorphic Ethanol Sedation Sensitivity inFrontiers in neural circuits · 2022Article
- white regulates proliferative homeostasis of intestinal stem cells during ageing in Drosophila.Nature metabolism · 2021Article
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- Sensitivity of Allelic Divergence to Genomic Position: Lessons from the Drosophila tan Gene.G3 (Bethesda, Md.) · 2016Article
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- Contrasting influences of Drosophila white/mini-white on ethanol sensitivity in two different behavioral assays.Alcoholism, clinical and experimental research · 2014Article
- Study of functional activity of the 1A2 insulator and polyadenylation signal in intron of the yellow gene of Drosophila melanogaster.Doklady. Biochemistry and biophysics · 2014Article
- Disruption of the protein kinase N gene of drosophila melanogaster results in the recessive delorean allele (pkndln) with a negative impact on wing morphogenesis.G3 (Bethesda, Md.) · 2014Article
- Transcription through enhancers suppresses their activity in Drosophila.Epigenetics & chromatin · 2013Article
- New properties of Drosophila scs and scs' insulators.PloS one · 2013Article
- Drosophila errantiviruses.Mobile genetic elements · 2012Article
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- The Functions and Mechanisms of Action of Insulators in the Genomes of Higher Eukaryotes.Acta naturaeArticle
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
The white gene, which is responsible for eye pigmentation, is widely used to study position effects in Drosophila. As a result of insertion of P-element vectors containing mini-white without enhancers into random chromosomal sites, flies with different eye color phenotypes appear, which is usually explained by the influence of positive/negative regulatory elements located around the insertion site. We found that, in more than 70% of cases when mini-white expression was subject to positive position effects, deletion of the white promoter had no effect on eye pigmentation; in these cases, the transposon was inserted into the transcribed regions of genes. Therefore, transcription through the mini-white gene could be responsible for high levels of its expression in most of chromosomal sites. Consistently with this conclusion, transcriptional terminators proved to be efficient in protecting mini-white expression from positive position effects. On the other hand, the best characterized Drosophila gypsy insulator was poorly effective in terminating transcription and, as a consequence, only partially protected mini-white expression from these effects. Thus, to ensure maximum protection of a transgene from position effects, a perfect boundary/insulator element should combine three activities: to block enhancers, to provide a barrier between active and repressed chromatin, and to terminate transcription.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.