Evidence map›Paper›PMID 19854928›Full record

ArticleApplied and environmental microbiology2010

Effect of glycosylation and additional domains on the thermostability of a family 10 xylanase produced by Thermopolyspora flexuosa.

Sasikala Anbarasan, Janne Jänis, Marja Paloheimo, Mikko Laitaoja, Minna Vuolanto, Johanna Karimäki, Pirjo Vainiotalo, Matti Leisola, Ossi Turunen

Abstract read
In one paragraph

Article in Applied and environmental microbiology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.

0numbers the graph read from it
0cells of the map it votes in
13citing papers in PubMed
1.6field-weighted citation impact, top 16% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

13 citing papers in PubMed, 45 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 2 institutions in 1 country.

Sasikala AnbarasanDepartment of Biotechnology and Chemical Technology, Aalto University, P.O. Box 6100, 02015 TKK, Espoo, Finland.
Janne Jänis
Marja Paloheimo
Mikko Laitaoja
Minna Vuolanto
Johanna Karimäki
Pirjo Vainiotalo
Matti Leisola
Ossi Turunen
Aalto University · FIRoal (Finland) · FI

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The effects of different structural features on the thermostability of Thermopolyspora flexuosa xylanase XYN10A were investigated. A C-terminal carbohydrate binding module had only a slight effect, whereas a polyhistidine tag increased the thermostability of XYN10A xylanase. In contrast, glycosylation at Asn26, located in an exposed loop, decreased the thermostability of the xylanase. The presence of a substrate increased stability mainly at low pH.

Indexed as

ActinomycetalesEndo-1,4-beta XylanasesEnzyme StabilityGlycosylationHot TemperatureHydrogen-Ion ConcentrationModels, MolecularProtein Structure, TertiaryEndo-1,4-beta Xylanases

Identifiers

PMID19854928
PMCPMC2798631
OpenAlexW2167611972

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.