ArticleJournal of bacteriology2010
Metabolic control of virulence genes in Brucella abortus: HutC coordinates virB expression and the histidine utilization pathway by direct binding to both promoters.
Article in Journal of bacteriology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers.
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Who cites it
42 citing papers in PubMed, 66 citations in OpenAlex.
- Disruption of Histidine Biosynthesis Impairs Outer Membrane Stability and Intracellular Survival ofMicroorganisms · 2026Article
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- A previously uncharacterized Fur-family metalloregulator integrates iron- and manganese-sensing to control virulence gene regulatory networks in Brucella.Scientific reports · 2025Article
- Breaking the silence-role of MucR as a virulence determinant inJournal of bacteriology · 2025Review
- Type I-E* CRISPR-Cas ofmSphere · 2025Article
- Quorum sensing LuxR proteins VjbR and BabR jointly regulateJournal of bacteriology · 2025Article
- ThemicroLife · 2025Review
- Increased Brucella abortus asRNA_0067 expression under intraphagocytic stressors is associated with enhanced virB2 transcription.Archives of microbiology · 2024Article
- Unraveling the Role of the Zinc-Dependent Metalloproteinase/HTH-Xre Toxin/Antitoxin (TA) System ofToxins · 2023Article
- Structural and Functional Characterization of Rv0792c from Mycobacterium tuberculosis: Identifying Small Molecule Inhibitor against HutC Protein.Microbiology spectrum · 2023Article
- Article
- Brucella melitensis invA gene (BME_RS01060) transcription is promoted under acidic stress conditions.Archives of microbiology · 2021Article
- The VirB System Plays a Crucial Role inInternational journal of molecular sciences · 2021Review
- An Extracytoplasmic Function Sigma/Anti-Sigma Factor System Regulates Hypochlorous Acid Resistance and Impacts Expression of the Type IV Secretion System inJournal of bacteriology · 2021Article
- A Novel Marine Pathogen Isolated from Wild Cunners (Microorganisms · 2021Article
- Uncovering the Hidden Credentials ofMicrobiology and molecular biology reviews : MMBR · 2021Review
- Comparative Genome-Wide Transcriptome Analysis ofFrontiers in microbiology · 2021Article
- Cell membrane components of Brucella melitensis play important roles in the resistance of low-level rifampicin.PLoS neglected tropical diseases · 2020Article
- Adhesins ofPathogens (Basel, Switzerland) · 2020Review
- Histidine Utilization Is a Critical Determinant ofInfection and immunity · 2020Article
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Authors and funding
5 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Type IV secretion systems (T4SS) are multicomponent machineries involved in the translocation of effector molecules across the bacterial cell envelope. The virB operon of Brucella abortus codes for a T4SS that is essential for virulence and intracellular multiplication of the bacterium in the host. Previous studies showed that the virB operon of B. abortus is tightly regulated within the host cells. In order to identify factors implicated in the control of virB expression, we searched for proteins of Brucella that directly bind to the virB promoter (P(virB)). Using different procedures, we isolated a 27-kDa protein that binds specifically to P(virB). This protein was identified as HutC, the transcriptional repressor of the histidine utilization (hut) genes. Analyses of virB and hut promoter activity revealed that HutC exerts two different roles: it acts as a coactivator of transcription of the virB operon, whereas it represses the hut genes. Such activities were observed both intracellularly and in bacteria incubated under conditions that resemble the intracellular environment. Electrophoresis mobility shift assays (EMSA) and DNase I footprinting experiments revealed the structure, affinity, and localization of the HutC-binding sites and supported the regulatory role of HutC in both hut and virB promoters. Taken together, these results indicate that Brucella coopted the function of HutC to coordinate the Hut pathway with transcriptional regulation of the virB genes, probably as a way to sense its own metabolic state and develop adaptive responses to overcome intracellular host defenses.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.