ArticleJournal of bacteriology2010
Transcriptional and functional analysis of the Neisseria gonorrhoeae Fur regulon.
Article in Journal of bacteriology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 43 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
43 citing papers in PubMed, 61 citations in OpenAlex.
- Microevolution of Neisseria lactamica during nasopharyngeal colonisation induced by controlled human infection.Nature communications · 2018Trial
- Genomic analysis ofMicrobiology spectrum · 2026Article
- Restriction-modification systems are required forProceedings of the National Academy of Sciences of the United States of America · 2026Article
- Norepinephrine derepresses the Fur regulon ofJournal of bacteriology · 2026Article
- Dual species transcriptomics reveals conserved metabolic and immunologic processes in interactions between human neutrophils and Neisseria gonorrhoeae.PLoS pathogens · 2024Article
- Transcriptional activation ofmBio · 2023Article
- Metal piracy by Neisseria gonorrhoeae to overcome human nutritional immunity.PLoS pathogens · 2023Article
- Thiostrepton, a resurging drug inhibiting the stringent response to counteract antibiotic-resistance and expression of virulence determinants inFrontiers in microbiology · 2023Article
- Molecular Regulatory Mechanisms Drive Emergent Pathogenetic Properties ofMicroorganisms · 2022Review
- Stealthy microbes: HowFrontiers in cellular and infection microbiology · 2022Review
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- Structure of the RecombinantmSphere · 2018Article
- Subversion of nutritional immunity by the pathogenic Neisseriae.Pathogens and disease · 2018Review
- The ironclad truth: how in vivo transcriptomics and in vitro mechanistic studies shape our understanding of Neisseria gonorrhoeae gene regulation during mucosal infection.Pathogens and disease · 2017Review
- Iron-regulated small RNA expression as Neisseria gonorrhoeae FA 1090 transitions into stationary phase growth.BMC genomics · 2017Article
- Article
- Characterization of the Neisseria gonorrhoeae Iron and Fur Regulatory Network.Journal of bacteriology · 2016Article
- Proteomics-driven Antigen Discovery for Development of Vaccines Against Gonorrhea.Molecular & cellular proteomics : MCP · 2016Article
- The iron stimulon and fur regulon of Geobacter sulfurreducens and their role in energy metabolism.Applied and environmental microbiology · 2014Article
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 1 country.
Funding
Abstract
To ensure survival in the host, bacteria have evolved strategies to acquire the essential element iron. In Neisseria gonorrhoeae, the ferric uptake regulator Fur regulates metabolism through transcriptional control of iron-responsive genes by binding conserved Fur box (FB) sequences in promoters during iron-replete growth. Our previous studies showed that Fur also controls the transcription of secondary regulators that may, in turn, control pathways important to pathogenesis, indicating an indirect role for Fur in controlling these downstream genes. To better define the iron-regulated cascade of transcriptional control, we combined three global strategies--temporal transcriptome analysis, genomewide in silico FB prediction, and Fur titration assays (FURTA)--to detect genomic regions able to bind Fur in vivo. The majority of the 300 iron-repressed genes were predicted to be of unknown function, followed by genes involved in iron metabolism, cell communication, and intermediary metabolism. The 107 iron-induced genes encoded hypothetical proteins or energy metabolism functions. We found 28 predicted FBs in FURTA-positive clones in the promoters and within the open reading frames of iron-repressed genes. We found lower levels of conservation at critical thymidine residues involved in Fur binding in the FB sequence logos of FURTA-positive clones with intragenic FBs than in the sequence logos generated from FURTA-positive promoter regions. In electrophoretic mobility shift assay studies, intragenic FBs bound Fur with a lower affinity than intergenic FBs. Our findings further indicate that transcription under iron stress is indirectly controlled by Fur through 12 potential secondary regulators.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.