ArticleJournal of bacteriology2010
Structure and regulation of the gab gene cluster, involved in the gamma-aminobutyric acid shunt, are controlled by a sigma54 factor in Bacillus thuringiensis.
Article in Journal of bacteriology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 33 citations in OpenAlex.
- From Gut Microbiomes to Infectious Pathogens: Neurological Disease Game Changers.Molecular neurobiology · 2025Review
- Enhanced accumulation of γ-aminobutyric acid by deletion of aminotransferase genes involved in γ-aminobutyric acid catabolism in engineeredApplied and environmental microbiology · 2024Article
- Transcriptional regulation of cellobiose utilization by PRD-domain containing Sigma54-dependent transcriptional activator (CelR) and catabolite control protein A (CcpA) inFrontiers in microbiology · 2024Article
- Role of the gut-microbiota-metabolite axis in the rotenone model of early-stage Parkinson's Disease.Metabolic brain disease · 2022Article
- C4 Bacterial Volatiles Improve Plant Health.Pathogens (Basel, Switzerland) · 2021Review
- Gut microbial molecules in behavioural and neurodegenerative conditions.Nature reviews. Neuroscience · 2020Review
- Gut microbiome signatures of nursing home residents carrying Enterobacteria producing extended-spectrum β-lactamases.Antimicrobial resistance and infection control · 2020Article
- Regulation of γ-Aminobutyrate (GABA) Utilization inFrontiers in microbiology · 2020Article
- Sox transcription in sarcosine utilization is controlled by Sigma(54) and SoxR in Bacillus thuringiensis HD73.Scientific reports · 2016Article
- Homology-Based Modeling of Universal Stress Protein fromFrontiers in microbiology · 2016Article
- Article
- Identification of metabolism pathways directly regulated by sigma(54) factor in Bacillus thuringiensis.Frontiers in microbiology · 2015Article
- Activation of gab cluster transcription in Bacillus thuringiensis by γ-aminobutyric acid or succinic semialdehyde is mediated by the Sigma 54-dependent transcriptional activator GabR.BMC microbiology · 2014Article
- Transcription of the lysine-2,3-aminomutase gene in the kam locus of Bacillus thuringiensis subsp. kurstaki HD73 is controlled by both σ54 and σK factors.Journal of bacteriology · 2014Article
- Review
- The metabolic regulation of sporulation and parasporal crystal formation in Bacillus thuringiensis revealed by transcriptomics and proteomics.Molecular & cellular proteomics : MCP · 2013Article
- Comparative analyses imply that the enigmatic Sigma factor 54 is a central controller of the bacterial exterior.BMC genomics · 2011Article
- Multiple controls affect arsenite oxidase gene expression in Herminiimonas arsenicoxydans.BMC microbiology · 2010Article
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Authors and funding
7 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The structure and regulation of the gab gene cluster, involved in gamma-aminobutyric acid (GABA) shunt, were studied by characterizing gabT and gabD genes cloned from Bacillus thuringiensis. Deletions of the gabT and gabD genes in B. thuringiensis strain HD-73 did not affect the growth of mutant strains in rich culture media, but the growth of a gabT deletion mutant strain was reduced in basic media (containing 0.2% GABA). Genome analysis indicates that the structure of the gab gene cluster in B. thuringiensis HD-73 is different from that in Escherichia coli and Bacillus subtilis but is common in strains of the Bacillus cereus group. This suggests that the gene cluster involved in GABA shunt is specific to the B. cereus group. Based on reverse transcription-PCR and transcriptional fusion analysis, we confirmed that the gabT and gabD genes belong to different transcriptional units, while the gabD and gabR genes form an operon. We also demonstrated that the gabR gene plays a positive regulatory role in gabD and gabT expression. The GabR protein may be a sigma(54)-dependent transcriptional activator, according to a conserved domain search in the NCBI database, and it is highly conserved in the B. cereus group. The -24/-12 consensus sequence of a promoter upstream from gabT suggests that the promoter can be recognized by a sigma(54) factor. Further analysis of the genetic complementation studies also suggests that the expression of the gabT gene is controlled by a sigma(54) factor. Thus, the expression of the gab cluster is regulated by a sigma(54) factor by way of the transcription activator GabR.
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