ArticleTransgenic research2010
Arabidopsis thaliana Rubisco small subunit transit peptide increases the accumulation of Thermotoga maritima endoglucanase Cel5A in chloroplasts of transgenic tobacco plants.
Article in Transgenic research, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 56 citations in OpenAlex.
- The transgenic Vip3A poplar plant confers high resistance against Hyphantria cunea Drury.Transgenic research · 2026Article
- Identification of a highly efficient chloroplast-targeting peptide for plastid engineering.PLoS biology · 2024Article
- The N-region sequence context impacts the chloroplast import efficiency of multi-TMD protein.Plant molecular biology · 2024Article
- Initiation of B-type starch granules in wheat endosperm requires the plastidial α-glucan phosphorylase PHS1.The Plant cell · 2023Article
- An optimized transformation protocol for Anthoceros agrestis and three more hornwort species.The Plant journal : for cell and molecular biology · 2023Article
- Salt stress-induced chloroplastic hydrogen peroxide stimulates pdTPI sulfenylation and methylglyoxal accumulation.The Plant cell · 2023Article
- Increasing the Efficiency of the Accumulation of Recombinant Proteins in Plant Cells: The Role of Transport Signal Peptides.Plants (Basel, Switzerland) · 2022Review
- Production ofFrontiers in plant science · 2021Article
- Overexpression of a multifunctional β-glucosidase gene from thermophilic archaeon Sulfolobus solfataricus in transgenic tobacco could facilitate glucose release and its use as a reporter.Transgenic research · 2020Article
- Use of synthetic biology tools to optimize the production of active nitrogenase Fe protein in chloroplasts of tobacco leaf cells.Plant biotechnology journal · 2020Article
- Production of recombinant proteins through sequestration in chloroplasts: a strategy based on nuclear transformation and post-translational protein import.Plant cell reports · 2019Review
- Reporter-based forward genetic screen to identify bundle sheath anatomy mutants in A. thaliana.The Plant journal : for cell and molecular biology · 2019Article
- LHCSR Expression under HSP70/RBCS2 Promoter as a Strategy to Increase Productivity in Microalgae.International journal of molecular sciences · 2018Article
- An optimized transit peptide for effective targeting of diverse foreign proteins into chloroplasts in rice.Scientific reports · 2017Article
- In planta production and characterization of a hyperthermostable GH10 xylanase in transgenic sugarcane.Plant molecular biology · 2017Article
- Article
- Strategies for the production of cell wall-deconstructing enzymes in lignocellulosic biomass and their utilization for biofuel production.Plant biotechnology journal · 2016Review
- Cell wall modification in tobacco by differential targeting of recombinant endoglucanase from Trichoderma reesei.BMC plant biology · 2015Article
- Phenotypic Changes in Transgenic Tobacco Plants Overexpressing Vacuole-Targeted Thermotoga maritima BglB Related to Elevated Levels of Liberated Hormones.Frontiers in bioengineering and biotechnology · 2015Article
- Cytonuclear evolution of rubisco in four allopolyploid lineages.Molecular biology and evolution · 2014Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Over the past decade various approaches have been used to increase the expression level of recombinant proteins in plants. One successful approach has been to target proteins to specific subcellular sites/compartments of plant cells, such as the chloroplast. In the study reported here, hyperthermostable endoglucanase Cel5A was targeted into the chloroplasts of tobacco plants via the N-terminal transit peptide of nuclear-encoded plastid proteins. The expression levels of Cel5A transgenic lines were then determined using three distinct transit peptides, namely, the light-harvesting chlorophyll a/b-binding protein (CAB), Rubisco small subunit (RS), and Rubisco activase (RA). RS:Cel5A transgenic lines produced highly stable active enzymes, and the protein accumulation of these transgenic lines was up to 5.2% of the total soluble protein in the crude leaf extract, remaining stable throughout the life cycle of the tobacco plant. Transmission election microscopy analysis showed that efficient targeting of Cel5A protein was under the control of the transit peptide.
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