ArticlePloS one2009
Identification, RNAi knockdown, and functional analysis of an ejaculate protein that mediates a postmating, prezygotic phenotype in a cricket.
Article in PloS one, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
27 citing papers in PubMed, 73 citations in OpenAlex.
- Experimental Evidence That Prenatal and Postnatal Developmental Stress Affects the Adult Seminal Fluid Proteome in a Precocial Bird.Molecular ecology · 2026Article
- Maturation of Eupyrene Sperm upon Ejaculation Is Influenced by a Male Accessory Gland-Derived Serine Protease inInsects · 2025Article
- Synthesis and Scope of the Role of Postmating Prezygotic Isolation in Speciation.Cold Spring Harbor perspectives in biology · 2024Review
- Genome and Genetic Engineering of the House Cricket (Biomolecules · 2023Article
- Article
- The carboxypeptidase B and carbonic anhydrase genes play a reproductive regulatory role during multiple matings inFrontiers in molecular biosciences · 2023Article
- CRISPR/Cas9-mediatedFrontiers in physiology · 2022Article
- Proteolysis in Reproduction: Lessons From Gene-Modified Organism Studies.Frontiers in endocrinology · 2022Review
- Comprehensive dynamic analysis of proteins in the spermatheca of female Haemaphysalis longicornis after copulation.Experimental & applied acarology · 2021Article
- PCE3 Plays a Role in the Reproduction of MaleInsects · 2021Article
- Male Age Influences Re-mating Incidence and Sperm Use in Females of the Dengue VectorFrontiers in physiology · 2021Article
- Seminal fluid and accessory male investment in sperm competition.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2020Review
- Transcriptome analysis of life stages of the house cricket, Acheta domesticus, to improve insect crop production.Scientific reports · 2020Article
- Seminal fluid-mediated fitness effects in the simultaneously hermaphroditic flatwormEcology and evolution · 2019Article
- Positive selection and comparative molecular evolution of reproductive proteins from New Zealand tree weta (Orthoptera, Hemideina).PloS one · 2017Article
- Molecular characterization of firefly nuptial gifts: a multi-omics approach sheds light on postcopulatory sexual selection.Scientific reports · 2016Article
- Article
- Combining RNA-seq and proteomic profiling to identify seminal fluid proteins in the migratory grasshopper Melanoplus sanguinipes (F).BMC genomics · 2015Article
- Hybrid male sterility between Drosophila willistoni species is caused by male failure to transfer sperm during copulation.BMC evolutionary biology · 2015Article
- Identification and function of proteolysis regulators in seminal fluid.Molecular reproduction and development · 2013Review
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Postmating, prezygotic phenotypes, especially those that underlie reproductive isolation between closely related species, have been a central focus of evolutionary biologists over the past two decades. Such phenotypes are thought to evolve rapidly and be nearly ubiquitous among sexually reproducing eukaryotes where females mate with multiple partners. Because these phenotypes represent interplay between the male ejaculate and female reproductive tract, they are fertile ground for reproductive senescence--as ejaculate composition and female physiology typically change over an individual's life span. Although these phenotypes and their resulting dynamics are important, we have little understanding of the proteins that mediate these phenotypes, particularly for species groups where postmating, prezygotic traits are the primary mechanism of reproductive isolation. Here, we utilize proteomics, RNAi, mating experiments, and the Allonemobius socius complex of crickets, whose members are primarily isolated from one another by postmating, prezygotic phenotypes (including the ability of a male to induce a female to lay eggs), to demonstrate that one of the most abundant ejaculate proteins (a male accessory gland-biased protein similar to a trypsin-like serine protease) decreases in abundance over a male's reproductive lifetime and mediates the induction of egg-laying in females. These findings represent one of the first studies to identify a protein that plays a role in mediating both a postmating, prezygotic isolation pathway and reproductive senescence.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.