ArticleProceedings of the National Academy of Sciences of the United States of America2009
Ligand migration through the internal hydrophobic cavities in human neuroglobin.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 50 citations in OpenAlex.
- Neuroglobin: A New Player in the Gut-Brain Axis.International journal of molecular sciences · 2026Review
- Kinetic and dynamical properties of truncated hemoglobins of the Antarctic bacterium Pseudoalteromonas haloplanktis TAC125.Protein science : a publication of the Protein Society · 2024Article
- NO and Heme Proteins: Cross-Talk between Heme and Cysteine Residues.Antioxidants (Basel, Switzerland) · 2023Review
- Probing the Role of Murine Neuroglobin CDloop-D-Helix Unit in CO Ligand Binding and Structural Dynamics.ACS chemical biology · 2022Article
- Structural and (Pseudo-)Enzymatic Properties of Neuroglobin: Its Possible Role in Neuroprotection.Cells · 2021Review
- Unusually FastInternational journal of molecular sciences · 2021Article
- Ligand pathways in neuroglobin revealed by low-temperature photodissociation and docking experiments.IUCrJ · 2019Article
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- Neuroglobin Expression in the Brain: a Story of Tissue Homeostasis Preservation.Molecular neurobiology · 2019Review
- Mapping Hydrophobic Tunnels and Cavities in Neuroglobin with Noble Gas under Pressure.Biophysical journal · 2017Article
- Determinants of neuroglobin plasticity highlighted by joint coarse-grained simulations and high pressure crystallography.Scientific reports · 2017Article
- Neuroglobin, a Factor Playing for Nerve Cell Survival.International journal of molecular sciences · 2016Review
- Heme orientation modulates histidine dissociation and ligand binding kinetics in the hexacoordinated human neuroglobin.Journal of biological inorganic chemistry : JBIC : a publication of the Society of Biological Inorganic Chemistry · 2013Article
- CO rebinding kinetics and molecular dynamics simulations highlight dynamic regulation of internal cavities in human cytoglobin.PloS one · 2013Article
- Filtering artifacts from lifetime distributions when maximizing entropy using a bootstrapped model.Analytical biochemistry · 2012Article
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- Article
- Following ligand migration pathways from picoseconds to milliseconds in type II truncated hemoglobin from Thermobifida fusca.PloS one · 2012Article
- 14-3-3 binding and phosphorylation of neuroglobin during hypoxia modulate six-to-five heme pocket coordination and rate of nitrite reduction to nitric oxide.The Journal of biological chemistry · 2011Article
- Probing the role of the internal disulfide bond in regulating conformational dynamics in neuroglobin.Biophysical journal · 2010Article
Corrections and comments
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Authors and funding
8 authors at 5 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Neuroglobin (Ngb), a member of the globin superfamily, was found in the brain of vertebrates and is suggested to play a neuroprotective function under hypoxic conditions by scavenging nitrogen monoxide (NO) through a dioxygenase activity. In order for such a reaction to efficiently take place and to minimize the release of reactive intermediates in the cytosol, the cosubstrates O(2) and NO and other unstable reaction intermediates should bind sequentially to docking sites in the protein matrix. We have characterized the accessibility of these sites by analyzing the geminate CO rebinding kinetics to the heme moiety observed upon nanosecond flash photolysis of the Ngb-CO complex encapsulated in silica gels. The geminate rebinding phase showed a remarkable complexity, revealing the presence of a system of secondary docking sites where ligands are stored for hundreds of microseconds. Most kinetics steps display little temperature dependence, demonstrating that ligands can easily migrate through the cavities, except for the slowest reaction intermediate, possibly reflecting a structural conformational change reshaping the system of cavities. This conformational change is unrelated with distal His E7 binding to the heme, as it persists for the HE7L mutant. Overall, data are consistent with the presence of a discrete system of docking sites, possibly acting as reservoirs for the putative cosubstrates and for other reactive species involved in the physiologically relevant reaction.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.