ArticleJournal of microbiological methods2009
A self-deleting Cre-lox-ermAM cassette, Cheshire, for marker-less gene deletion in Streptococcus pneumoniae.
Article in Journal of microbiological methods, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
19 citing papers in PubMed.
- A Small Non-Coding RNA Modulates Expression of Pilus-1 Type inMicroorganisms · 2021Article
- New Insights into Beta-Lactam Resistance ofMicroorganisms · 2021Article
- Lox'd in translation: contradictions in the nomenclature surrounding common lox-site mutants and their implications in experiments.Microbiology (Reading, England) · 2021Article
- The pneumococcal σThe Journal of biological chemistry · 2019Article
- Genomics and Genetics ofMicrobiology spectrum · 2019Review
- Transcriptional organization of pneumococcal psrP-secY2A2 and impact of GtfA and GtfB deletion on PsrP-associated virulence properties.Microbes and infection · 2017Article
- A Novel Vector for Construction of Markerless Multicopy Overexpression Transformants inFrontiers in microbiology · 2017Article
- ManLMN is a glucose transporter and central metabolic regulator in Streptococcus pneumoniae.Molecular microbiology · 2016Article
- Competence for Genetic Transformation in Streptococcus pneumoniae: Mutations in σA Bypass the ComW Requirement for Late Gene Expression.Journal of bacteriology · 2016Article
- Carbon catabolite repression by seryl phosphorylated HPr is essential to Streptococcus pneumoniae in carbohydrate-rich environments.Molecular microbiology · 2015Article
- The aspartate-less receiver (ALR) domains: distribution, structure and function.PLoS pathogens · 2015Article
- Control of competence by related non-coding csRNAs in Streptococcus pneumoniae R6.Frontiers in genetics · 2015Article
- LocZ is a new cell division protein involved in proper septum placement in Streptococcus pneumoniae.mBio · 2014Article
- Competence for genetic transformation in Streptococcus pneumoniae: mutations in σA bypass the comW requirement.Journal of bacteriology · 2014Article
- Mobilizable Rolling-Circle Replicating Plasmids from Gram-Positive Bacteria: A Low-Cost Conjugative Transfer.Microbiology spectrum · 2014Article
- Adenylate kinase from Streptococcus pneumoniae is essential for growth through its catalytic activity.FEBS open bio · 2014Article
- Inactivated pep27 mutant as an effective mucosal vaccine against a secondary lethal pneumococcal challenge in mice.Clinical and experimental vaccine research · 2013Article
- Decrease in penicillin susceptibility due to heat shock protein ClpL in Streptococcus pneumoniae.Antimicrobial agents and chemotherapy · 2011Article
- Development of a versatile procedure based on natural transformation for marker-free targeted genetic modification in Streptococcus thermophilus.Applied and environmental microbiology · 2010Article
Corrections and comments
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Authors and funding
3 authors.
Funding
Abstract
Although targeted mutagenesis of Streptococcus pneumoniae is readily accomplished with the aid of natural genetic transformation and chimeric donor DNA constructs assembled in vitro, the drug resistance markers often employed for selection of recombinant products can themselves be undesirable by-products of the genetic manipulation. A new cassette carrying the erythromycin-resistance marker ermAM that can be used as a temporary marker for selection of desired recombinants is described. The cassette may subsequently be removed at will by virtue of an embedded fucose-regulated Cre recombinase gene and terminal lox66 and lox71 Cre recognition sites, with retention of 34bp from the cassette as an inert residual double-mutant lox72 site.
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Registered trials
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