ArticleMolecular microbiology2009
Reconstructing the evolutionary history of nitrotoluene detection in the transcriptional regulator NtdR.
Article in Molecular microbiology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
15 citing papers in PubMed.
- Evolution of pollutant biodegradation.Applied microbiology and biotechnology · 2025Review
- Evolution ofApplied and environmental microbiology · 2024Article
- Article
- Molecular Basis and Evolutionary Origin of 1-Nitronaphthalene Catabolism inApplied and environmental microbiology · 2023Article
- Biodegradation of 3-Chloronitrobenzene and 3-Bromonitrobenzene byApplied and environmental microbiology · 2022Article
- Constitutive Expression of a Nag-Like Dioxygenase Gene through an Internal Promoter in the 2-Chloronitrobenzene Catabolism Gene Cluster of Pseudomonas stutzeri ZWLR2-1.Applied and environmental microbiology · 2016Article
- The Highly ConservedFrontiers in microbiology · 2016Article
- Genetically engineered microorganisms for the detection of explosives' residues.Frontiers in microbiology · 2015Review
- Selection for growth on 3-nitrotoluene by 2-nitrotoluene-utilizing Acidovorax sp. strain JS42 identifies nitroarene dioxygenases with altered specificities.Applied and environmental microbiology · 2015Article
- Stepwise enhancement of catalytic performance of haloalkane dehalogenase LinB towards β-hexachlorocyclohexane.AMB Express · 2014Article
- Three types of taxis used in the response of Acidovorax sp. strain JS42 to 2-nitrotoluene.Applied and environmental microbiology · 2012Article
- Directed evolution of the transcriptional regulator DntR: isolation of mutants with improved DNT-response.PloS one · 2012Article
- Evolution of a new bacterial pathway for 4-nitrotoluene degradation.Molecular microbiology · 2011Article
- Factors that influence the response of the LysR type transcriptional regulators to aromatic compounds.BMC biochemistry · 2011Article
- Nitroaromatic compounds, from synthesis to biodegradation.Microbiology and molecular biology reviews : MMBR · 2010Review
Corrections and comments
- Commented on by
Authors and funding
3 authors.
Funding
Abstract
Many toxic man-made compounds have been introduced into the environment, and bacterial strains that are able to grow on them are ideal model systems for studying the evolution of metabolic pathways and regulatory systems. Acidovorax sp. strain JS42 is unique in its ability to use 2-nitrotoluene as a sole carbon, nitrogen, and energy source for growth. The LysR-type transcriptional regulator NtdR activates expression of the 2-nitrotoluene degradation genes not only when nitroaromatic compounds are present, but also in the presence of a wide range of aromatic acids and analogues. The molecular determinants of inducer specificity were identified through comparative analysis with NagR, the activator of the naphthalene degradation pathway genes in Ralstonia sp. strain U2. Although NagR is 98% identical to NtdR, it does not respond to nitrotoluenes. Exchange of residues that differ between NagR and NtdR revealed that residues at positions 227 and 232 were key for the recognition of nitroaromatic compounds, while the amino acid at position 169 determined the range of aromatic acids recognized. Structural modelling of NtdR suggests that these residues are near the predicted inducer binding pocket. Based on these results, an evolutionary model is presented that depicts the stepwise evolution of NtdR.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.