ArticleProceedings of the National Academy of Sciences of the United States of America2010
Interrogating functional integration between injected pluripotent stem cell-derived cells and surrogate cardiac tissue.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
38 citing papers in PubMed, 94 citations in OpenAlex.
- Elastomeric Polyesters in Cardiovascular Tissue Engineering and Organs-on-a-Chip.Biomacromolecules · 2023Review
- FRESH 3D bioprinting a contractile heart tube using human stem cell-derived cardiomyocytes.Biofabrication · 2022Article
- A comprehensive analysis of gene expression changes in a high replicate and open-source dataset of differentiating hiPSC-derived cardiomyocytes.Scientific reports · 2021Article
- Harnessing organs-on-a-chip to model tissue regeneration.Cell stem cell · 2021Review
- Considerations to Model Heart Disease in Women with Preeclampsia and Cardiovascular Disease.Cells · 2021Review
- Optimizing the Use of iPSC-CMs for Cardiac Regeneration in Animal Models.Animals : an open access journal from MDPI · 2020Review
- The Heart and Great Vessels.Cold Spring Harbor perspectives in medicine · 2018Review
- Article
- Human Induced Pluripotent Stem Cells Differentiate Into Functional Mesenchymal Stem Cells and Repair Bone Defects.Stem cells translational medicine · 2016Article
- Maturing human pluripotent stem cell-derived cardiomyocytes in human engineered cardiac tissues.Advanced drug delivery reviews · 2016Review
- Tissue engineering the cardiac microenvironment: Multicellular microphysiological systems for drug screening.Advanced drug delivery reviews · 2016Review
- Formation of vascular network structures within cardiac cell sheets from mouse embryonic stem cells.Regenerative therapy · 2015Article
- Evolutionarily conserved intercalated disc protein Tmem65 regulates cardiac conduction and connexin 43 function.Nature communications · 2015Article
- Enhanced Electrical Integration of Engineered Human Myocardium via Intramyocardial versus Epicardial Delivery in Infarcted Rat Hearts.PloS one · 2015Article
- The role of tissue engineering and biomaterials in cardiac regenerative medicine.The Canadian journal of cardiology · 2014Review
- Cardiac regeneration using pluripotent stem cells--progression to large animal models.Stem cell research · 2014Review
- Advancing functional engineered cardiac tissues toward a preclinical model of human myocardium.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2014Article
- Design and formulation of functional pluripotent stem cell-derived cardiac microtissues.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- Current status of induced pluripotent stem cells in cardiac tissue regeneration and engineering.Regenerative medicine research · 2013Review
- Cell-based therapy for the deficient urinary sphincter.Current urology reports · 2013Review
Corrections and comments
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Authors and funding
12 authors at 5 institutions in 3 countries.
Funding
Abstract
Myocardial infarction resulting in irreversible loss of cardiomyocytes (CMs) remains a leading cause of heart failure. Although cell transplantation has modestly improved cardiac function, major challenges including increasing cell survival, engraftment, and functional integration with host tissue, remain. Embryonic stem cells (ESCs), which can be differentiated into cardiac progenitors (CPs) and CMs, represent a candidate cell source for cardiac cell therapy. However, it is not known what specific cell type or condition is the most appropriate for transplantation. This problem is exasperated by the lack of efficient and predictive strategies to screen the large numbers of parameters that may impact cell transplantation. We used a cardiac tissue model, engineered heart tissue (EHT), and quantitative molecular and electrophysiological analyses, to test transplantation conditions and specific cell populations for their potential to functionally integrate with the host tissue. In this study, we validated our analytical platform using contractile mouse neonatal CMs (nCMs) and noncontractile cardiac fibroblasts (cFBs), and screened for the integration potential of ESC-derived CMs and CPs (ESC-CMs and -CPs). Consistent with previous in vivo studies, cFB injection interfered with electrical signal propagation, whereas injected nCMs improved tissue function. Purified bioreactor-generated ESC-CMs exhibited a diminished capacity for electrophysiological integration; a result correlated with lower (compared with nCMs) connexin 43 expression. ESC-CPs, however, appeared able to appropriately mature and integrate into EHT, enhancing the amplitude of tissue contraction. Our results support the use of EHT as a model system to accelerate development of cardiac cell therapy strategies.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.