ArticleMolecular biology of the cell2009
Bub3p facilitates spindle checkpoint silencing in fission yeast.
Article in Molecular biology of the cell, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
16 citing papers in PubMed, 30 citations in OpenAlex.
- Generation of a Spindle Checkpoint Arrest from Synthetic Signaling Assemblies.Current biology : CB · 2017Article
- Cryo-EM of Mitotic Checkpoint Complex-Bound APC/C Reveals Reciprocal and Conformational Regulation of Ubiquitin Ligation.Molecular cell · 2016Article
- Two functionally distinct kinetochore pools of BubR1 ensure accurate chromosome segregation.Nature communications · 2016Article
- Mps1Mph1 Kinase Phosphorylates Mad3 to Inhibit Cdc20Slp1-APC/C and Maintain Spindle Checkpoint Arrests.PLoS genetics · 2016Article
- The mitotic checkpoint complex (MCC): looking back and forth after 15 years.AIMS molecular science · 2016Article
- Distinct domains in Bub1 localize RZZ and BubR1 to kinetochores to regulate the checkpoint.Nature communications · 2015Article
- Bimodal activation of BubR1 by Bub3 sustains mitotic checkpoint signaling.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- Checkpoint proteins come under scrutiny.eLife · 2013Article
- Bub3 reads phosphorylated MELT repeats to promote spindle assembly checkpoint signaling.eLife · 2013Article
- MPS1/Mph1 phosphorylates the kinetochore protein KNL1/Spc7 to recruit SAC components.Nature cell biology · 2012Article
- Phosphodependent recruitment of Bub1 and Bub3 to Spc7/KNL1 by Mph1 kinase maintains the spindle checkpoint.Current biology : CB · 2012Article
- Centromere-tethered Mps1 pombe homolog (Mph1) kinase is a sufficient marker for recruitment of the spindle checkpoint protein Bub1, but not Mad1.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- Nsk1 ensures accurate chromosome segregation by promoting association of kinetochores to spindle poles during anaphase B.Molecular biology of the cell · 2011Article
- Spindle checkpoint silencing requires association of PP1 to both Spc7 and kinesin-8 motors.Developmental cell · 2011Article
- Ringing the changes: emerging roles for DASH at the kinetochore-microtubule Interface.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2011Review
- Overcoming inhibition in the spindle checkpoint.Genes & development · 2009Review
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
Although critical for spindle checkpoint signaling, the role kinetochores play in anaphase promoting complex (APC) inhibition remains unclear. Here we show that spindle checkpoint proteins are severely depleted from unattached kinetochores in fission yeast cells lacking Bub3p. Surprisingly, a robust mitotic arrest is maintained in the majority of bub3 Delta cells, yet they die, suggesting that Bub3p is essential for successful checkpoint recovery. During recovery, two defects are observed: (1) cells mis-segregate chromosomes and (2) anaphase onset is significantly delayed. We show that Bub3p is required to activate the APC upon inhibition of Aurora kinase activity in checkpoint-arrested cells, suggesting that Bub3p is required for efficient checkpoint silencing downstream of Aurora kinase. Together, these results suggest that spindle checkpoint signals can be amplified in the nucleoplasm, yet kinetochore localization of spindle checkpoint components is required for proper recovery from a spindle checkpoint-dependent arrest.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.