ArticleJournal of clinical microbiology2009
(CGG)4-based PCR as a novel tool for discrimination of uropathogenic Escherichia coli strains: comparison with enterobacterial repetitive intergenic consensus-PCR.
Article in Journal of clinical microbiology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 54 citations in OpenAlex.
- Characterisation of Typical Enteropathogenic Escherichia coli (tEPEC) and bfpA Genes From Five Bat Species Reveals Australian Pteropus Spp. Are Natural tEPEC Hosts.MicrobiologyOpen · 2026Article
- UropathogenicInternational journal of molecular sciences · 2025Article
- Article
- Comparative study of virulence potential, phylogenetic origin, CRISPR-Frontiers in microbiology · 2023Article
- Molecular Characterization of Extended Spectrum β-Lactamase (ESBL) and Virulence Gene-Factors in UropathogenicAntibiotics (Basel, Switzerland) · 2022Article
- High Prevalence of Beta-Lactam-ResistantMicroorganisms · 2022Article
- Molecular and metabolic characteristics of wastewater associated Escherichia coli strains.Environmental microbiology reports · 2022Article
- Molecular analysis of Pseudomonas aeruginosa strains isolated from cystic fibrosis patients.Scientific reports · 2021Article
- Comparison of Antimicrobial Resistance, Virulence Genes, Phylogroups, and Biofilm Formation ofFrontiers in microbiology · 2021Article
- Article
- Pathogenic Factors Correlate With Antimicrobial Resistance Among ClinicalFrontiers in microbiology · 2020Article
- Ciprofloxacin, amoxicillin, and aminoglycosides stimulate genetic and phenotypic changes in uropathogenic Escherichia coli strains.Virulence · 2019Article
- Numerical interpretation of TRS-PCR profiling results for Escherichia coli strains isolated from patients with bacteriuria in Lodz region, Poland.Molecular biology reports · 2019Article
- Article
- Effect of Dietary Copper on Intestinal Microbiota and Antimicrobial Resistance Profiles ofFrontiers in microbiology · 2019Article
- The genetic background of antibiotic resistance among clinical uropathogenic Escherichia coli strains.Molecular biology reports · 2018Article
- Predicting pathogenicity behavior in Escherichia coli population through a state dependent model and TRS profiling.PLoS computational biology · 2018Article
- Article
- A new look at the drug-resistance investigation of uropathogenic E. coli strains.Molecular biology reports · 2017Article
- TRS-PCR profiling for discrimination of Escherichia coli strains isolated from children with diarrhea under 5 years of age in Lodz region, Poland.Molecular biology reports · 2016Article
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Urinary tract infections are one of the most frequent bacterial diseases in humans, and Escherichia coli is most often the relevant pathogen. A specific pathotype of E. coli, known as uropathogenic E. coli (UPEC), often causes serious and difficult-to-treat infections of the urinary tract. We propose a new single-tube screening tool that uses an (N)(6)(CGG)(4) primer to generate fingerprint profiles that allow rapid discrimination and epidemiology of this group of bacteria. We found 71 different CGG-PCR profiles among 127 E. coli strains, while enterobacterial repetitive intergenic consensus (ERIC)-PCR of the same group yielded only 28 profiles. Additionally, the (CGG)(4)-based PCR test turned out to be very effective for clustering UPEC strains exhibiting multiple virulence genes and usually belonging to the B2 phylogenetic group, and it separated these strains from E. coli strains lacking most of the UPEC-specific virulence factors. Since the reproducibility of the CGG-PCR screen is higher than that of ERIC-PCR, our test should be a valuable means of increasing the discriminatory power of current UPEC typing schemes.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.