ArticleThe Journal of biological chemistry2009
Characterization of the key step for light-driven hydrogen evolution in green algae.
Article in The Journal of biological chemistry, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Who cites it
32 citing papers in PubMed, 124 citations in OpenAlex.
- Article
- Energy metabolism and adaptation to hypoxia in the non-photosynthetic green alga Leontynka.BMC biology · 2026Article
- Hydrogen-Producing Catalysts Based on Ferredoxin Scaffolds.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Light-Driven HPlants (Basel, Switzerland) · 2024Review
- The AlgaInternational journal of molecular sciences · 2023Article
- A Novel Oncogenic Role of FDX1 in Human Melanoma Related to PD-L1 Immune Checkpoint.International journal of molecular sciences · 2023Article
- Molecular Basis of the Electron Bifurcation Mechanism in the [FeFe]-Hydrogenase Complex HydABC.Journal of the American Chemical Society · 2023Article
- Changing the tracks: screening for electron transfer proteins to support hydrogen production.Journal of biological inorganic chemistry : JBIC : a publication of the Society of Biological Inorganic Chemistry · 2022Article
- Second and Outer Coordination Sphere Effects in Nitrogenase, Hydrogenase, Formate Dehydrogenase, and CO Dehydrogenase.Chemical reviews · 2022Review
- Re-routing photosynthetic energy for continuous hydrogen production in vivo.Biotechnology for biofuels · 2019Article
- Absolute quantification of selected photosynthetic electron transfer proteins in Chlamydomonas reinhardtii in the presence and absence of oxygen.Photosynthesis research · 2018Article
- Green Algal Hydrogenase Activity Is Outcompeted by Carbon Fixation before Inactivation by Oxygen Takes Place.Plant physiology · 2018Article
- Evolution of Chlamydomonas reinhardtii ferredoxins and their interactions with [FeFe]-hydrogenases.Photosynthesis research · 2017Review
- Frequency and potential dependence of reversible electrocatalytic hydrogen interconversion by [FeFe]-hydrogenases.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- Temperature-sensitive PSII: a novel approach for sustained photosynthetic hydrogen production.Photosynthesis research · 2016Article
- Predicting Protein-Protein Interactions Using BiGGER: Case Studies.Molecules (Basel, Switzerland) · 2016Article
- Crystal structure and biochemical characterization of Chlamydomonas FDX2 reveal two residues that, when mutated, partially confer FDX2 the redox potential and catalytic properties of FDX1.Photosynthesis research · 2016Article
- Identification of the Elusive Pyruvate Reductase of Chlamydomonas reinhardtii Chloroplasts.Plant & cell physiology · 2016Article
- Implementation of photobiological H2 production: the O 2 sensitivity of hydrogenases.Photosynthesis research · 2015Article
- Role of the mitochondrial alternative oxidase pathway in hydrogen photoproduction in Chlorella protothecoides.Planta · 2015Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Under anaerobic conditions, several species of green algae perform a light-dependent hydrogen production catalyzed by a special group of [FeFe] hydrogenases termed HydA. Although highly interesting for biotechnological applications, the direct connection between photosynthetic electron transport and hydrogenase activity is still a matter of speculation. By establishing an in vitro reconstitution system, we demonstrate that the photosynthetic ferredoxin (PetF) is essential for efficient electron transfer between photosystem I and HydA1. To investigate the electrostatic interaction process and electron transfer between PetF and HydA1, we performed site-directed mutagenesis. Kinetic analyses with several site-directed mutagenesis variants of HydA1 and PetF enabled us to localize the respective contact sites. These experiments in combination with in silico docking analyses indicate that electrostatic interactions between the conserved HydA1 residue Lys(396) and the C terminus of PetF as well as between the PetF residue Glu(122) and the N-terminal amino group of HydA1 play a major role in complex formation and electron transfer. Mapping of relevant HydA1 and PetF residues constitutes an important basis for manipulating the physiological photosynthetic electron flow in favor of light-driven H(2) production.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.