ArticlePlant physiology2009
Arabidopsis FAB1/PIKfyve proteins are essential for development of viable pollen.
Article in Plant physiology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 50 papers.
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Who cites it
50 citing papers in PubMed, 112 citations in OpenAlex.
- Phosphatidylinositol-4-phosphate signaling regulates dense granule biogenesis and maturation in Toxoplasma gondii.PLoS pathogens · 2026Article
- JULGI coordinates vascular development and leaf patterning through non-cell-autonomous regulation of miR165/166.Nature communications · 2026Article
- OsSNDP4, a Sec14-nodulin Domain Protein, is Required for Pollen Development in Rice.Rice (New York, N.Y.) · 2024Article
- FAB1C, a phosphatidylinositol 3-phosphate 5-kinase, interacts with PIN-FORMEDs and modulates their lytic trafficking in Arabidopsis.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Review
- Regulation of phosphoinositide metabolism in Apicomplexan parasites.Frontiers in cell and developmental biology · 2023Review
- The PH Domain and C-Terminal polyD Motif of Phafin2 Exhibit a Unique Concurrence in Animals.Membranes · 2022Article
- Male Fertility under Environmental Stress: Do Polyamines Act as Pollen Tube Growth Protectants?International journal of molecular sciences · 2022Review
- Tonoplast proton pumps regulate nuclear spacing of female gametophytesFrontiers in plant science · 2022Article
- Two Is Company, but Four Is a Party-Challenges of Tetraploidization for Cell Wall Dynamics and Efficient Tip-Growth in Pollen.Plants (Basel, Switzerland) · 2021Review
- Using high-throughput multiple optical phenotyping to decipher the genetic architecture of maize drought tolerance.Genome biology · 2021Article
- An FYVE-Domain-Containing Protein, PsFP1, Is Involved in Vegetative Growth, Oxidative Stress Response and Virulence ofInternational journal of molecular sciences · 2021Article
- The canonical α-SNAP is essential for gametophytic development in Arabidopsis.PLoS genetics · 2021Article
- Functions and Mechanisms of SAC Phosphoinositide Phosphatases in Plants.Frontiers in plant science · 2021Review
- Loss of PIKfyve Causes Transdifferentiation ofFrontiers in cell and developmental biology · 2021Article
- Transcriptome Co-expression Network Analysis Identifies Key Genes Regulating Conchosporangia Maturation ofFrontiers in genetics · 2021Article
- AUTOPHAGY-RELATED14 and Its Associated Phosphatidylinositol 3-Kinase Complex Promote Autophagy in Arabidopsis.The Plant cell · 2020Article
- Ubiquitination of interleukin-1α is associated with increased pro-inflammatory polarization of murine macrophages deficient in the E3 ligase ITCH.The Journal of biological chemistry · 2020Article
- COPII Components Sar1b and Sar1c Play Distinct Yet Interchangeable Roles in Pollen Development.Plant physiology · 2020Article
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Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Phosphatidylinositol 3,5-bisphosphate [PtdIns(3,5)P(2)] is a phospholipid that has a role in controlling membrane trafficking events in yeast and animal cells. The function of this lipid in plants is unknown, although its synthesis has been shown to be up-regulated upon osmotic stress in plant cells. PtdIns(3,5)P(2) is synthesized by the PIKfyve/Fab1 family of proteins, with two orthologs, FAB1A and FAB1B, being present in Arabidopsis (Arabidopsis thaliana). In this study, we attempt to address the role of this lipid by analyzing the phenotypes of plants mutated in FAB1A and FAB1B. It was not possible to generate plants homozygous for mutations in both genes, although single mutants were isolated. Both homozygous single mutant plant lines exhibited a leaf curl phenotype that was more marked in FAB1B mutants. Genetic transmission analysis revealed that failure to generate double mutant lines was entirely due to inviability of pollen carrying mutant alleles of both FAB1A and FAB1B. This pollen displayed severe defects in vacuolar reorganization following the first mitotic division of development. The presence of abnormally large vacuoles in pollen at the tricellular stage resulted in the collapse of the majority of grains carrying both mutant alleles. This demonstrates a crucial role for PtdIns(3,5)P(2) in modulating the dynamics of vacuolar rearrangement essential for successful pollen development. Taken together, our results are consistent with PtdIns(3,5)P(2) production being central to cellular responses to changes in osmotic conditions.
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