ArticleBMC cell biology2009
Novel association of APC with intermediate filaments identified using a new versatile APC antibody.
Article in BMC cell biology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 28 citations in OpenAlex.
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- (Pro)renin receptor promotes colorectal cancer progression through inhibiting the NEDD4L-mediated Wnt3 ubiquitination and modulating gut microbiota.Cell communication and signaling : CCS · 2023Article
- Branched actin networks are assembled on microtubules by adenomatous polyposis coli for targeted membrane protrusion.The Journal of cell biology · 2020Article
- Oncogenic Serine 45-Deleted β-Catenin Remains Susceptible to Wnt Stimulation and APC Regulation in Human Colonocytes.Cancers · 2020Article
- APC controls Wnt-induced β-catenin destruction complex recruitment in human colonocytes.Scientific reports · 2020Article
- Validation and application of a novel APC antibody in western blotting, immunoprecipitation, and immunohistochemistry.Medical molecular morphology · 2018Article
- Multi-scale modeling of APC and [Formula: see text]-catenin regulation in the human colonic crypt.Journal of mathematical biology · 2018Article
- Insulin signaling regulates a functional interaction between adenomatous polyposis coli and cytoplasmic dynein.Molecular biology of the cell · 2017Article
- Suppression of intestinal tumorigenesis inJournal of cell science · 2017Article
- The APC tumor suppressor is required for epithelial cell polarization and three-dimensional morphogenesis.Biochimica et biophysica acta · 2015Article
- An APC:WNT Counter-Current-Like Mechanism Regulates Cell Division Along the Human Colonic Crypt Axis: A Mechanism That Explains How APC Mutations Induce Proliferative Abnormalities That Drive Colon Cancer Development.Frontiers in oncology · 2013Review
- APC binds intermediate filaments and is required for their reorganization during cell migration.The Journal of cell biology · 2013Article
- APC/β-catenin-rich complexes at membrane protrusions regulate mammary tumor cell migration and mesenchymal morphology.BMC cancer · 2013Article
- A knock-in mouse model reveals roles for nuclear Apc in cell proliferation, Wnt signal inhibition and tumor suppression.Oncogene · 2012Article
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- Changes in gene expression and cellular architecture in an ovarian cancer progression model.PloS one · 2011Article
- Novel double-negative feedback loop between adenomatous polyposis coli and Musashi1 in colon epithelia.The Journal of biological chemistry · 2011Article
- Adenomatous Polyposis Coli (APC) in cell migration.European journal of cell biologyReview
- Mass Spectrometry-Based Proteomics Technologies to Define Endogenous Protein-Protein Interactions and Their Applications to Cancer and Viral Infectious Diseases.Mass spectrometry reviewsReview
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
backgroundAs a key player in suppression of colon tumorigenesis, Adenomatous Polyposis Coli (APC) has been widely studied to determine its cellular functions. However, inconsistencies of commercially available APC antibodies have limited the exploration of APC function. APC is implicated in spindle formation by direct interactions with tubulin and microtubule-binding protein EB1. APC also interacts with the actin cytoskeleton to regulate cell polarity. Until now, interaction of APC with the third cytoskeletal element, intermediate filaments, has remained unexamined.
resultsWe generated an APC antibody (APC-M2 pAb) raised against the 15 amino acid repeat region, and verified its reliability in applications including immunoprecipitation, immunoblotting, and immunofluorescence in cultured cells and tissue. Utilizing this APC-M2 pAb, we immunoprecipitated endogenous APC and its binding proteins from colon epithelial cells expressing wild-type APC. Using Liquid Chromatography Tandem Mass Spectrometry (LC-MS/MS), we identified 42 proteins in complex with APC, including beta-catenin and intermediate filament (IF) proteins lamin B1 and keratin 81. Association of lamin B1 with APC in cultured cells and human colonic tissue was verified by co-immunoprecipitation and colocalization. APC also colocalized with keratins and remained associated with IF proteins throughout a sequential extraction procedure.
conclusionWe introduce a versatile APC antibody that is useful for cell/tissue immunostaining, immunoblotting and immunoprecipitation. We also present evidence for interactions between APC and IFs, independent of actin filaments and microtubules. Our results suggest that APC associates with all three major components of the cytoskeleton, thus expanding potential roles for APC in the regulation of cytoskeletal integrity.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.