ArticleProtein science : a publication of the Protein Society2009
An interdomain disulfide bridge links the NtA and first FS domain in agrin.
Article in Protein science : a publication of the Protein Society, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
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5 citing papers in PubMed.
- Dissecting the Extracellular Complexity of Neuromuscular Junction Organizers.Frontiers in molecular biosciences · 2019Review
- Interaction studies of a protein and carbohydrate system using an integrated approach: a case study of the miniagrin-heparin system.European biophysics journal : EBJ · 2018Article
- Structural mechanisms of the agrin-LRP4-MuSK signaling pathway in neuromuscular junction differentiation.Cellular and molecular life sciences : CMLS · 2013Review
- Article
- T-shaped arrangement of the recombinant agrin G3-IgG Fc protein.Protein science : a publication of the Protein Society · 2011Article
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2 authors.
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Abstract
Agrin is a multidomain heparan sulfate proteoglycan involved in postsynaptic differentiation at the neuromuscular junction. Binding of agrin to synaptic basal lamina is mediated by the N-terminal agrin (NtA) domain. The NtA domain of agrin is followed by a tandem of nine follistatin-like (FS) domains forming a rod-like spacer to the laminin G-like domains of the molecule. Here we report that the most C-terminal cysteine residue of NtA (Cys123) forms an interdomain disulfide bond with the FOLN subdomain of the FS module. Remarkably, this single cysteine is flanked by Leu117 and Val124, which are two essential beta-branched amino acids forming the heterocomplex of NtA with the gamma 1 chain of laminin. Moreover, we show that this covalent linkage compensates for the seven amino acid residue splice insert at the very C-terminal helix H3 and causes a rigid interface between NtA and FS independent of the alternative mRNA splice event. These results suggest that the interdomain disulfide bond between the NtA and the first FS domain might be important for the proper folding of agrin.
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