ArticleBiophysical journal2009
Two-path impedance spectroscopy for measuring paracellular and transcellular epithelial resistance.
Article in Biophysical journal, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers.
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Who cites it
41 citing papers in PubMed.
- Modular Integration of Impedance Sensing for Real-Time Assessment of Barrier Integrity.bioRxiv : the preprint server for biology · 2026Article
- Stretchable Porous Membranes for Barrier Tissue Models with Real-Time Measurement and Biomimetic Cyclic Strain.Micromachines · 2025Article
- Flow-induced mechano-modulation of intestinal permeability on chip.Materials today. Bio · 2025Article
- The PAR2 Antagonist Larazotide Can Mitigate Acute Histamine-Stimulated Epithelial Barrier Disruption in Keratinocytes: A Potential Adjunct Treatment for Atopic Dermatitis.JID innovations : skin science from molecules to population health · 2025Article
- Pinoresinol enhances oral barrier integrity and function in human buccal cell monolayers.PloS one · 2025Article
- Integrating Continuous Transepithelial Flux Measurements into an Ussing Chamber Set-Up.International journal of molecular sciences · 2024Article
- A biologically validated mathematical model for decoding epithelial apical, basolateral, and paracellular electrical properties.American journal of physiology. Cell physiology · 2023Article
- Ag- but Not ZnO-Nanoparticles Disturb the Airway Epithelial Barrier at Subtoxic Concentrations.Pharmaceutics · 2023Article
- Role of Muscarinic Acetylcholine Receptors in Intestinal Epithelial Homeostasis: Insights for the Treatment of Inflammatory Bowel Disease.International journal of molecular sciences · 2023Review
- Angulin-1 (LSR) Affects Paracellular Water Transport, However Only in Tight Epithelial Cells.International journal of molecular sciences · 2021Article
- Transendothelial Electrical Resistance Measurement across the Blood-Brain Barrier: A Critical Review of Methods.Micromachines · 2021Review
- Reprogramming Intestinal Epithelial Cell Polarity by Interleukin-22.Frontiers in medicine · 2021Article
- The Role of Intestinal Permeability in Gastrointestinal Disorders and Current Methods of Evaluation.Frontiers in nutrition · 2021Review
- Diarrheal Mechanisms and the Role of Intestinal Barrier Dysfunction in Campylobacter Infections.Current topics in microbiology and immunology · 2021Article
- Plasmonic-based impedance microspectroscopy of optically heterogeneous samples.Biomedical optics express · 2020Article
- Two-Stage Interpretation of Changes in TEER of Intestinal Epithelial Layers Protected by Adhering Bifidobacteria DuringFrontiers in microbiology · 2020Article
- Tilivalline- and Tilimycin-Independent Effects ofInternational journal of molecular sciences · 2019Article
- An isogenic hiPSC-derived BBB-on-a-chip.Biomicrofluidics · 2019Article
- Application of Permeation Enhancers in Oral Delivery of Macromolecules: An Update.Pharmaceutics · 2019Review
- Impedance Spectroscopy as a Tool for Monitoring Performance in 3D Models of Epithelial Tissues.Frontiers in bioengineering and biotechnology · 2019Review
Corrections and comments
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Solutes are transported across epithelial cell layers via transcellular and paracellular pathways. The transcellular pathway leads across the apical and basolateral cell membrane, whereas the paracellular pathway is directed through the tight junction. Tight junction proteins (claudins, occludin, tricellulin) can not only form barriers but also paracellular channels that are--in concert with membrane channels and transporters--regulated in a wide range in health and disease states. Thus, it is desirable to determine para- and transcellular resistance (R(para), R(trans)) separately. This cannot be achieved by conventional transepithelial resistance (TER) measurements. We present an impedance spectroscopic approach that is optimized for differentiation between these two pathways. The method is based on a transepithelial impedance measurement in specialized Ussing chambers, combined with a Ca(2+)-dependent modulation of R(para) through EGTA and flux measurements of a nonradioactive paracellular marker, fluorescein. The prerequisites are a paracellular marker that varies in parallel to 1/R(para), an experimental regime that alters R(para) without affecting R(trans), and exact knowledge of the resistance of subepithelial components. The underlying prerequisites and the applicability as a routine method were verified on cell lines of different tightness including HT-29/B6 colon cells and Madin-Darby canine kidney tubule cells C7 and C11.
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