ArticleBiophysical journal2009
Spatial distribution and mobility of the Ran GTPase in live interphase cells.
Article in Biophysical journal, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
13 citing papers in PubMed, 20 citations in OpenAlex.
- Diversity in emergent cell locomotion from the coupling cytosolic and cortical Marangoni flows with reaction-diffusion dynamics.PLoS computational biology · 2026Article
- Infectious bursal disease virus VP5 triggers host shutoff in a transcription-dependent manner.mBio · 2024Article
- Using FCS to accurately measure protein concentration in the presence of noise and photobleaching.Biophysical journal · 2021Article
- Article
- A Chemomechanical Model for Nuclear Morphology and Stresses during Cell Transendothelial Migration.Biophysical journal · 2016Article
- Article
- Volume regulation and shape bifurcation in the cell nucleus.Journal of cell science · 2015Article
- A systematic comparison of mathematical models for inherent measurement of ciliary length: how a cell can measure length and volume.Biophysical journal · 2015Article
- The proapoptotic protein tBid forms both superficially bound and membrane-inserted oligomers.Biophysical journal · 2014Article
- Single-molecule studies of nucleocytoplasmic transport: from one dimension to three dimensions.Integrative biology : quantitative biosciences from nano to macro · 2012Review
- Development of time-integrated multipoint moment analysis for spatially resolved fluctuation spectroscopy with high time resolution.Biophysical journal · 2011Article
- Single molecule studies of nucleocytoplasmic transport.Biochimica et biophysica acta · 2011Review
- Fluorescence correlation spectroscopy of intact nuclear pore complexes.Biophysical journal · 2011Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
Abstract
The GTPase Ran is a key regulator of molecular transport through nuclear pore complex (NPC) channels. To analyze the role of Ran in its nuclear transport function, we used several quantitative fluorescence techniques to follow the distribution and dynamics of an enhanced yellow fluorescent protein (EYFP)-Ran in HeLa cells. The diffusion coefficient of the majority of EYFP-Ran molecules throughout the cells corresponded to an unbound state, revealing the remarkably dynamic Ran regulation. Although we observed no significant immobile Ran populations in cells, approximately 10% of the cytoplasmic EYFP-Ran and 30% of the nuclear EYFP-Ran exhibited low mobility indicative of molecular interactions. The high fraction of slow nuclear EYFP-Ran reflects the expected numerous interactions of nuclear RanGTP with nuclear transport receptors. However, it is not high enough to support retention mechanisms as the main cause for the observed nuclear accumulation of Ran. The highest cellular concentration of EYFP-Ran was detected at the nuclear envelope, corresponding to approximately 200 endogenous Ran molecules for each NPC, and exceeding the currently estimated NPC channel transport capacity. Together with the relatively long residence time of EYFP-Ran at the nuclear envelope (33 +/- 14 ms), these observations suggest that only a fraction of the Ran concentrated at NPCs transits through NPC channels.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.