ArticleCurrent microbiology2010
Functional identification of a putative beta-galactosidase gene in the special lac gene cluster of Lactobacillus acidophilus.
Article in Current microbiology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 19 citations in OpenAlex.
- Valorization of whey-based side streams for microbial biomass, molecular hydrogen, and hydrogenase production.Applied microbiology and biotechnology · 2023Article
- Probiotic potential of β‑galactosidase‑producing lactic acid bacteria from fermented milk and their molecular characterization.Biomedical reports · 2023Article
- Cloning, Expression, Purification, and Characterization of β-Galactosidase fromMolecules (Basel, Switzerland) · 2022Article
- Determining the Role of UTP-Glucose-1-Phosphate Uridylyltransferase (GalU) in Improving the Resistance ofFoods (Basel, Switzerland) · 2022Article
- Nucleic acid lateral flow assay for simultaneous detection of hygiene indicator bacteria.Analytical and bioanalytical chemistry · 2021Article
- Construction and Analysis of Food-GradeJournal of microbiology and biotechnology · 2021Article
- Stimulatory effects of novel glucosylated lactose derivatives GL34 on growth of selected gut bacteria.Applied microbiology and biotechnology · 2019Article
- Surface Display of Heterologous β-Galactosidase in Food-Grade Recombinant Lactococcus lactis.Current microbiology · 2018Article
- Development and evaluation of an efficient heterologous gene knock-in reporter system in Lactococcus lactis.Microbial cell factories · 2017Article
- Kinetic studies on exploring lactose hydrolysis potential of β galactosidase extracted from Lactobacillus plantarum HF571129.Journal of food science and technology · 2015Article
- Preparation of lactose-free pasteurized milk with a recombinant thermostable β-glucosidase from Pyrococcus furiosus.BMC biotechnology · 2013Article
- Transcriptional and functional analysis of galactooligosaccharide uptake by lacS in Lactobacillus acidophilus.Proceedings of the National Academy of Sciences of the United States of America · 2011Article
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The putative beta-galactosidase gene (lacZ) of Lactobacillus acidophilus has a very low degree of homology to the Escherichia coli beta-galactosidase gene (lacZ) and locates in a special lac gene cluster which contains two beta-galactosidase genes. No functional characteristic of the putative beta-galactosidase has been described so far. In this study, the lacZ gene of L. acidophilus was hetero-expressed in E. coli and the recombinant protein was purified by a three-step procedure. The product of the lacZ gene was also extracted from L. acidophilus ATCC 4356 and active staining was carried out. The enzymatic properties of the purified recombinant LacZ were assayed. The results of hetero-expression showed the recombinant LacZ without tag had beta-galactosidase activity. The purified recombinant LacZ had a specific activity of 43.2 U/mg protein. The result of active staining showed that the functional product of the lacZ gene did exist in L. acidophilus. The L. acidophilus beta-galactosidase (LacZ) had an optimal pH of 6, an optimal temperature of 37 degrees C and could hydrolyze 73% of lactose in milk in 30 h at 10 degrees C. The L. acidophilus beta-galactosidase (LacZ) was identified as cold-adapted beta-galactosidase in this study for the first time, and may be useful for lactose removal from dairy products at low temperatures.
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