ArticleThe Journal of cell biology2009
Keratins regulate protein biosynthesis through localization of GLUT1 and -3 upstream of AMP kinase and Raptor.
Article in The Journal of cell biology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 72 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
72 citing papers in PubMed, 128 citations in OpenAlex.
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- One-Step Genetic Modification by Embryonic Doral Aorta Injection of Adenoviral CRISPR/Cas9 Vector in Chicken.International journal of molecular sciences · 2024Article
- Nuclear roles for non-lamin intermediate filament proteins.Current opinion in cell biology · 2024Review
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- The Adhesion G-Protein-Coupled Receptor GPR115/Cells · 2022Article
- Bidirectional regulation of desmosome hyperadhesion by keratin isotypes and desmosomal components.Cellular and molecular life sciences : CMLS · 2022Article
- Keratin filaments mediate the expansion of extra-embryonic membranes in the post-gastrulation mouse embryo.The EMBO journal · 2022Article
- Novel Diagnostic Biomarkers in Colorectal Cancer.International journal of molecular sciences · 2022Review
- Keratin 1 maintains the intestinal barrier in ulcerative colitis.Genes & genomics · 2021Article
- Keratins as an Inflammation Trigger Point in Epidermolysis Bullosa Simplex.International journal of molecular sciences · 2021Review
- Roles for Ndel1 in keratin organization and desmosome function.Molecular biology of the cell · 2021Article
- The Inclusion Principles of Human Embryos in the WOW-Based Time-Lapse System: A Retrospective Cohort Study.Frontiers in endocrinology · 2021Article
- An intact keratin network is crucial for mechanical integrity and barrier function in keratinocyte cell sheets.Cellular and molecular life sciences : CMLS · 2020Article
- Intestinal intermediate filament polypeptides in C. elegans: Common and isotype-specific contributions to intestinal ultrastructure and function.Scientific reports · 2020Article
- The keratin-desmosome scaffold: pivotal role of desmosomes for keratin network morphogenesis.Cellular and molecular life sciences : CMLS · 2020Article
- The interconnection between cytokeratin and cell membrane-bound β-catenin in Sertoli cells derived from juvenileBiology open · 2019Article
- Differential proteomic analysis revealed crucial egg white proteins for hatchability of chickens.Poultry science · 2019Article
12 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Keratin intermediate filament proteins form cytoskeletal scaffolds in epithelia, the disruption of which affects cytoarchitecture, cell growth, survival, and organelle transport. However, owing to redundancy, the global function of keratins has not been defined in full. Using a targeted gene deletion strategy, we generated transgenic mice lacking the entire keratin multiprotein family. In this study, we report that without keratins, embryonic epithelia suffer no cytolysis and maintain apical polarity but display mislocalized desmosomes. All keratin-null embryos die from severe growth retardation at embryonic day 9.5. We find that GLUT1 and -3 are mislocalized from the apical plasma membrane in embryonic epithelia, which subsequently activates the energy sensor adenosine monophosphate kinase (AMPK). Analysis of the mammalian target of rapamycin (mTOR) pathway reveals that AMPK induction activates Raptor, repressing protein biosynthesis through mTORC1's downstream targets S6 kinase and 4E-binding protein 1. Our findings demonstrate a novel keratin function upstream of mTOR signaling via GLUT localization and have implications for pathomechanisms and therapy approaches for keratin disorders and the analysis of other gene families.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.