ArticleInfection and immunity2010
CcpA and LacD.1 affect temporal regulation of Streptococcus pyogenes virulence genes.
Article in Infection and immunity, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 57 papers.
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Who cites it
57 citing papers in PubMed, 74 citations in OpenAlex.
- Lactate Oxidase (LctO) Acts as a Metabolic Checkpoint RestrainingbioRxiv : the preprint server for biology · 2026Article
- Mechanisms Underlying the Effects of Secretory ProteinMicroorganisms · 2025Article
- Dihydrothiazolo ring-fused 2-pyridone antimicrobial compounds treatScience advances · 2024Article
- Dihydrothiazolo ring-fused 2-pyridone antimicrobial compounds treatbioRxiv : the preprint server for biology · 2024Article
- Central carbon flux controls growth/damage balance for Streptococcus pyogenes.PLoS pathogens · 2023Article
- StkP- and PhpP-Mediated Posttranslational Modifications Modulate the S. pneumoniae Metabolism, Polysaccharide Capsule, and Virulence.Infection and immunity · 2023Article
- The role of CopA in Streptococcus pyogenes copper homeostasis and virulence.Journal of inorganic biochemistry · 2023Article
- The Role of Regulator Catabolite Control Protein A (CcpA) in Streptococcus agalactiae Physiology and Stress Response.Microbiology spectrum · 2022Article
- Deletion of lacD gene affected stress tolerance and virulence of Streptococcus suis serotype 2.Journal of microbiology (Seoul, Korea) · 2022Article
- Article
- Genome-wide analysis of in vivo CcpA binding with and without its key co-factor HPr in the major human pathogen group A Streptococcus.Molecular microbiology · 2021Article
- Subpopulation behaviors in lactose metabolism by Streptococcus mutans.Molecular microbiology · 2021Article
- Phosphotransferase System Uptake and Metabolism of the β-Glucoside Salicin Impact Group A Streptococcal Bloodstream Survival and Soft Tissue Infection.Infection and immunity · 2020Article
- Hydrogen Peroxide Production of Group A Streptococci (GAS) is emm-Type Dependent and Increased at Low Temperatures.Current microbiology · 2019Article
- DNA methylation from a Type I restriction modification system influences gene expression and virulence in Streptococcus pyogenes.PLoS pathogens · 2019Article
- CcpA Coordinates Growth/Damage Balance for Streptococcus pyogenes Pathogenesis.Scientific reports · 2018Article
- Glucose Levels Alter the Mga Virulence Regulon in the Group A Streptococcus.Scientific reports · 2018Article
- Route of Glucose Uptake in the Group a Streptococcus Impacts SLS-Mediated Hemolysis and Survival in Human Blood.Frontiers in cellular and infection microbiology · 2018Article
- Leaderless secreted peptide signaling molecule alters global gene expression and increases virulence of a human bacterial pathogen.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- Collagen-binding proteins of Streptococcus mutans and related streptococci.Molecular oral microbiology · 2017Review
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Production of H(2)O(2) follows a growth phase-dependent pattern that mimics that of many virulence factors of Streptococcus pyogenes. To gain greater insight into mechanisms coupling virulence factor expression to growth phase, we investigated the molecular basis for H(2)O(2) generation and its regulation. Deletion of the gene encoding lactate oxidase (lctO) or culture in the presence of glucose eliminated H(2)O(2) production, implicating carbohydrate regulation of lctO as a key element of growth phase control. In examining known carbohydrate-responsive regulators, deletion of the gene encoding CcpA but not that encoding LacD.1 resulted in both derepression and an uncoupling of lctO transcription from its growth phase pattern. Expanding this analysis to additional virulence factors demonstrated both negative (cfa, encoding CAMP factor) and positive (speB, encoding a cysteine protease) regulation by CcpA and that CcpA mutants were highly cytotoxic for cultured macrophages. This latter property resulted from enhanced transcription of the streptolysin S biogenesis operon. Examination of CcpA-promoter interactions using a DNA pull-down assay mimicking physiological conditions showed direct binding to the promoters of lctO and speB but not those of sagA. CcpA but not LacD.1 mutants were attenuated in a murine model of soft-tissue infection, and analysis of gene expression in infected tissue indicated that CcpA mutants had altered expression of lctO, cfa, and speB but not the indirectly regulated sagA gene. Taken together, these data show that CcpA regulates virulence genes via at least three distinct mechanisms and that disruption of growth phase regulation alters transcriptional patterns in infected tissues.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.