ArticleMolecular and cellular biology2010
TFIIF facilitates dissociation of RNA polymerase II from noncoding RNAs that lack a repression domain.
Article in Molecular and cellular biology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 19 citations in OpenAlex.
- Reading the SINEs - functionality in noncoding RNA 'cheap genes'.RNA biology · 2026Review
- Mechanism of mammalian transcriptional repression by noncoding RNA.Nature structural & molecular biology · 2025Article
- Exploring the secrets of long noncoding RNAs.International journal of molecular sciences · 2015Review
- Studying the affinity, kinetic stability, and specificity of RNA/protein interactions: SINE ncRNA/Pol II complexes as a model system.Methods in molecular biology (Clifton, N.J.) · 2015Article
- Structural insights into transcriptional repression by noncoding RNAs that bind to human Pol II.Journal of molecular biology · 2013Article
- The non-coding B2 RNA binds to the DNA cleft and active-site region of RNA polymerase II.Journal of molecular biology · 2013Article
- Struggling to let go: a non-coding RNA directs its own extension and destruction.The EMBO journal · 2013Article
- RNA polymerase II acts as an RNA-dependent RNA polymerase to extend and destabilize a non-coding RNA.The EMBO journal · 2013Article
- An integrated chemical cross-linking and mass spectrometry approach to study protein complex architecture and function.Molecular & cellular proteomics : MCP · 2012Article
- "Alu"strious long ncRNAs and their role in shortening mRNA half-lives.Cell cycle (Georgetown, Tex.) · 2011Article
- Genomic gems: SINE RNAs regulate mRNA production.Current opinion in genetics & development · 2010Review
- Article
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3 authors at 1 institution in 1 country.
Funding
Abstract
Noncoding RNAs (ncRNAs) have recently been found to regulate multiple steps in mammalian mRNA transcription. Mouse B2 RNA and human Alu RNA bind RNA polymerase II (Pol II) and repress mRNA transcription, using regions of the ncRNAs referred to as repression domains. Two other ncRNAs, mouse B1 RNA and human small cytoplasmic Alu (scAlu) RNA, bind Pol II with high affinity but lack repression domains and hence do not inhibit transcription. To better understand the interplay between ncRNAs that bind Pol II and their functions in transcription, we studied how Pol II binding and transcriptional repression are controlled by general transcription factors. We found that TFIIF associates with B1 RNA/Pol II and scAlu RNA/Pol II complexes and decreases their kinetic stability. Both subunits of TFIIF are required for this activity. Importantly, fusing a repression domain to B1 RNA stabilizes its interaction with Pol II in the presence of TFIIF. These results suggest a new role for TFIIF in regulating the interaction of ncRNAs with Pol II; specifically, it destabilizes interactions with ncRNAs that are not transcriptional repressors. These studies also identify a new function for ncRNA repression domains: they stabilize interactions of ncRNAs with Pol II in the presence of TFIIF.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.