ArticleJournal of cellular and molecular medicine2011
Glomerular parietal epithelial cells of adult murine kidney undergo EMT to generate cells with traits of renal progenitors.
Article in Journal of cellular and molecular medicine, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.
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Who cites it
40 citing papers in PubMed, 69 citations in OpenAlex.
- Conserved stromal EMT program controls regenerative capacity of mesenchymal stromal cells across multiple tissues.Science advances · 2026Article
- Crosstalk of injured podocytes with parietal epithelial cells through Wnt4/β-Catenin signaling.Scientific reports · 2025Article
- Transplantation of decellularized porcine kidney grafts repopulated with primary human cells demonstrates filtration function in pigs.Communications medicine · 2024Article
- The Role of the PAX Genes in Renal Cell Carcinoma.International journal of molecular sciences · 2024Review
- In vivo safety and biodistribution profile of Klotho-enhanced human urine-derived stem cells for clinical application.Stem cell research & therapy · 2023Article
- Single-copy Snail upregulation causes partial epithelial-mesenchymal transition in colon cancer cells.BMC cancer · 2023Article
- Differences in Immunohistochemical and Ultrastructural Features between Podocytes and Parietal Epithelial Cells (PECs) Are Observed in Developing, Healthy Postnatal, and Pathologically Changed Human Kidneys.International journal of molecular sciences · 2022Article
- Castrated autoimmune glomerulonephritis mouse model shows attenuated glomerular sclerosis with altered parietal epithelial cell phenotype.Experimental biology and medicine (Maywood, N.J.) · 2021Article
- Wnt signaling in kidney: the initiator or terminator?Journal of molecular medicine (Berlin, Germany) · 2020Review
- SIX1 Regulates Aberrant Endometrial Epithelial Cell Differentiation and Cancer Latency Following Developmental Estrogenic Chemical Exposure.Molecular cancer research : MCR · 2019Article
- Differential expression of parietal epithelial cell and podocyte extracellular matrix proteins in focal segmental glomerulosclerosis and diabetic nephropathy.American journal of physiology. Renal physiology · 2019Article
- Intermediate cell states in epithelial-to-mesenchymal transition.Physical biology · 2019Review
- Modeling of mesenchymal hybrid epithelial state and phenotypic transitions in EMT and MET processes of cancer cells.Scientific reports · 2018Article
- Shift of EMT gradient in 3D spheroid MSCs for activation of mesenchymal niche function.Scientific reports · 2017Article
- Activated ERK1/2 increases CD44 in glomerular parietal epithelial cells leading to matrix expansion.Kidney international · 2017Article
- Advances in the Knowledge about Kidney Decellularization and Repopulation.Frontiers in bioengineering and biotechnology · 2017Review
- Stability of the hybrid epithelial/mesenchymal phenotype.Oncotarget · 2016Article
- Tumor Budding: The Name is EMT. Partial EMT.Journal of clinical medicine · 2016Review
- Transforming growth factor-β1 and diabetic nephropathy.American journal of physiology. Renal physiology · 2016Review
- Emerging Transcriptional Mechanisms in the Regulation of Epithelial to Mesenchymal Transition and Cellular Plasticity in the Kidney.Journal of clinical medicine · 2016Review
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Glomerular parietal epithelial cells (GPECs) are known to revert to embryonic phenotype in response to renal injury. However, the mechanism of de-differentiation in GPECs and the underlying cellular processes are not fully understood. In the present study, we show that cultured GPECs of adult murine kidney undergo epithelial-mesenchymal transition (EMT) to generate cells, which express CD24, CD44 and CD29 surface antigens. Characterization by qRT-PCR and immunostaining of these clonogenic cells demonstrate that they exhibit metastable phenotype with co-expression of both epithelial (cytokeratin-18) and mesenchymal (vimentin) markers. Transcript analysis by qRT-PCR revealed high expression of metanephric mesenchymal (Pax-2, WT-1, Six-1, Eya-1, GDNF) and uteric bud (Hoxb-7, C-Ret) genes in these cells, indicating their bipotent progenitor status. Incubation of GPECs with EMT blocker Prostaglandin E2, resulted in low expression of renal progenitor markers reflecting the correlation between EMT and acquired stemness in these cells. Additional in vitro renal commitment assays confirmed their functional staminality. When injected into E13.5 kidney rudiments, the cells incorporated into the developing kidney primordia and co-culture with E13.5 spinal cord resulted in branching and tubulogenesis in these cells. When implanted under renal capsule of unilaterally nephrectomized mice, these cells differentiated into immature glomeruli and vascular ducts. Our study demonstrates that EMT plays a major role in imparting plasticity to terminally differentiated GPECs by producing metastable cells with traits of kidney progenitors. The present study would improve our understanding on epithelial cell plasticity, furthering our knowledge of its role in renal repair and regeneration.
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