ArticleJournal of cellular and molecular medicine2011
Aldehyde dehydrogenase activity promotes survival of human muscle precursor cells.
Article in Journal of cellular and molecular medicine, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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Who cites it
33 citing papers in PubMed, 59 citations in OpenAlex.
- Contractile myografts confer systemic anti-aging benefits.Nature aging · 2026Article
- ALDEFLUOR activity, ALDH isoforms, and their clinical significance in cancers.Journal of enzyme inhibition and medicinal chemistry · 2023Article
- Cancer Stem Cell Markers in Rhabdomyosarcoma in Children.Diagnostics (Basel, Switzerland) · 2022Article
- Article
- Bone-forming perivascular cells: Cellular heterogeneity and use for tissue repair.Stem cells (Dayton, Ohio) · 2021Article
- Identification of a Sesquiterpene Lactone fromMolecules (Basel, Switzerland) · 2021Article
- Aldehyde dehydrogenases contribute to skeletal muscle homeostasis in healthy, aging, and Duchenne muscular dystrophy patients.Journal of cachexia, sarcopenia and muscle · 2020Article
- Alda-1, an Aldehyde Dehydrogenase 2 Agonist, Improves Cutaneous Wound Healing by Activating Epidermal Keratinocytes via Akt/GSK-3β/β-Catenin Pathway.Aesthetic plastic surgery · 2020Article
- miR-206 family is important for mitochondrial and muscle function, but not essential for myogenesis in vitro.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2020Article
- Isolation and characterization of myogenic precursor cells from human cremaster muscle.Scientific reports · 2019Article
- Aldehyde Dehydrogenases: Not Just Markers, but Functional Regulators of Stem Cells.Stem cells international · 2019Review
- Article
- Identification of Newly Committed Pancreatic Cells in the Adult Mouse Pancreas.Scientific reports · 2017Article
- Redox Control of Skeletal Muscle Regeneration.Antioxidants & redox signaling · 2017Review
- Retinoic acid maintains human skeletal muscle progenitor cells in an immature state.Cellular and molecular life sciences : CMLS · 2017Article
- Reduced aldehyde dehydrogenase expression in preeclamptic decidual mesenchymal stem/stromal cells is restored by aldehyde dehydrogenase agonists.Scientific reports · 2017Article
- Nuclear respiratory factor 1 and endurance exercise promote human telomere transcription.Science advances · 2016Article
- Mesenchymal Stem/Stromal Cells Derived From a Reproductive Tissue Niche Under Oxidative Stress Have High Aldehyde Dehydrogenase Activity.Stem cell reviews and reports · 2016Article
- Aldehyde dehydrogenase 1A1 in stem cells and cancer.Oncotarget · 2016Review
- Key role of MEK/ERK pathway in sustaining tumorigenicity and in vitro radioresistance of embryonal rhabdomyosarcoma stem-like cell population.Molecular cancer · 2016Article
Corrections and comments
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Authors and funding
10 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Aldehyde dehydrogenases (ALDH) are a family of enzymes that efficiently detoxify aldehydic products generated by reactive oxygen species and might therefore participate in cell survival. Because ALDH activity has been used to identify normal and malignant cells with stem cell properties, we asked whether human myogenic precursor cells (myoblasts) could be identified and isolated based on their levels of ALDH activity. Human muscle explant-derived cells were incubated with ALDEFLUOR, a fluorescent substrate for ALDH, and we determined by flow cytometry the level of enzyme activity. We found that ALDH activity positively correlated with the myoblast-CD56(+) fraction in those cells, but, we also observed heterogeneity of ALDH activity levels within CD56-purified myoblasts. Using lentiviral mediated expression of shRNA we demonstrated that ALDH activity was associated with expression of Aldh1a1 protein. Surprisingly, ALDH activity and Aldh1a1 expression levels were very low in mouse, rat, rabbit and non-human primate myoblasts. Using different approaches, from pharmacological inhibition of ALDH activity by diethylaminobenzaldehyde, an inhibitor of class I ALDH, to cell fractionation by flow cytometry using the ALDEFLUOR assay, we characterized human myoblasts expressing low or high levels of ALDH. We correlated high ALDH activity ex vivo to resistance to hydrogen peroxide (H(2) O(2) )-induced cytotoxic effect and in vivo to improved cell viability when human myoblasts were transplanted into host muscle of immune deficient scid mice. Therefore detection of ALDH activity, as a purification strategy, could allow non-toxic and efficient isolation of a fraction of human myoblasts resistant to cytotoxic damage.
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Registered trials
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