ArticleNature biotechnology2009
Transcriptional analysis of intracytoplasmically stained, FACS-purified cells by high-throughput, quantitative nuclease protection.
Article in Nature biotechnology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed, 44 citations in OpenAlex.
- Stem/progenitor cells in normal physiology and disease of the pancreas.Molecular and cellular endocrinology · 2021Review
- Breakthrough in purification of fossil pollen for dating of sediments by a new large-particle on-chip sorter.Science advances · 2021Article
- FIN-Seq: transcriptional profiling of specific cell types from frozen archived tissue of the human central nervous system.Nucleic acids research · 2020Article
- Transcriptional Profiling of CD8+ CMV-Specific T Cell Functional Subsets Obtained Using a Modified Method for Isolating High-Quality RNA From Fixed and Permeabilized Cells.Frontiers in immunology · 2020Article
- Article
- Addressing cellular heterogeneity in tumor and circulation for refined prognostication.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- MafB Is Important for Pancreatic β-Cell Maintenance under a MafA-Deficient Condition.Molecular and cellular biology · 2019Article
- Examining How the MAFB Transcription Factor Affects Islet β-Cell Function Postnatally.Diabetes · 2019Article
- Notch signaling dynamically regulates adult β cell proliferation and maturity.The Journal of clinical investigation · 2019Article
- Interrogating islets in health and disease with single-cell technologies.Molecular metabolism · 2017Review
- An increase in immature β-cells lacking Glut2 precedes the expansion of β-cell mass in the pregnant mouse.PloS one · 2017Article
- Hsa-miR-24-3p increases nasopharyngeal carcinoma radiosensitivity by targeting both the 3'UTR and 5'UTR of Jab1/CSN5.Oncogene · 2016Article
- End Sequence Analysis Toolkit (ESAT) expands the extractable information from single-cell RNA-seq data.Genome research · 2016Article
- Gestational Diabetes Mellitus From Inactivation of Prolactin Receptor and MafB in Islet β-Cells.Diabetes · 2016Article
- Fixed single-cell transcriptomic characterization of human radial glial diversity.Nature methods · 2016Article
- Novel Observations From Next-Generation RNA Sequencing of Highly Purified Human Adult and Fetal Islet Cell Subsets.Diabetes · 2015Article
- The pancreatic β-cell transcriptome and integrated-omics.Current opinion in endocrinology, diabetes, and obesity · 2014Review
- In vivo molecular markers for pro-inflammatory cytokine M1 stage and resident microglia in trimethyltin-induced hippocampal injury.Neurotoxicity research · 2014Article
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Corrections and comments
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Authors and funding
9 authors at 4 institutions in 1 country.
Funding
Abstract
Analyzing specialized cells in heterogeneous tissues is crucial for understanding organ function in health and disease. Thus far, however, there has been no convenient method for studying gene expression in cells purified by fluorescence-activated cell sorting (FACS) using intracellular markers. Here we show that the quantitative nuclease protection assay (qNPA) enables transcriptional analysis of intracytoplasmically stained cells sorted by FACS. Applying the method to mouse pancreatic islet-cell subsets, we detected both expected and unknown lineage-specific gene expression patterns. Some beta cells from pregnant animals were found to express Mafb, previously observed only in immature beta cells during embryonic development. The four 'housekeeping' genes tested were expressed in purified islet-cell subpopulations with a notable variability, dependent on both cell lineage and developmental stage. Application of qNPA to intracellularly stained, FACS-sorted cells should be broadly applicable to the analysis of gene expression in subpopulations of any heterogeneous tissue, including tumors.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.