ArticleApplied and environmental microbiology2009
Optimization of a rapid viability assay for Mycobacterium avium subsp. paratuberculosis by using alamarBlue.
Article in Applied and environmental microbiology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed.
- Evaluation of the potential virulence of Mycobacterium avium subspecies paratuberculosis isolates from cattle in Uganda.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2026Article
- Review
- [Osteogenic differentiation of bone marrow mesenchymal stem cells induced by gene-loaded lipopolysaccharide-amine nanopolymersomes].Zhongguo xiu fu chong jian wai ke za zhi = Zhongguo xiufu chongjian waike zazhi = Chinese journal of reparative and reconstructive surgery · 2018Article
- 'Nano-immuno test' for the detection of live Mycobacterium avium subspecies paratuberculosis bacilli in the milk samples using magnetic nano-particles and chromogen.Veterinary research communications · 2018Article
- A Rapid Method for Quantifying Viable Mycobacterium avium subsp. paratuberculosis in Cellular Infection Assays.Applied and environmental microbiology · 2016Article
- Preparation and biological properties of ring-substituted naphthalene-1-carboxanilides.Molecules (Basel, Switzerland) · 2014Article
- Investigating the spectrum of biological activity of ring-substituted salicylanilides and carbamoylphenylcarbamates.Molecules (Basel, Switzerland) · 2010Article
- Gene encoded antimicrobial peptides, a template for the design of novel anti-mycobacterial drugs.Bioengineered bugsArticle
Corrections and comments
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Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A microtiter alamarBlue assay was adapted and optimized for Mycobacterium avium subsp. paratuberculosis. Using cell concentrations ranging from 10(4) to 10(8) CFU/ml, a minimum incubation time to indicate viability was obtained after 24 h. Rifampin (rifampicin) was used to demonstrate that this method has applications for high-throughput screening against M. avium subsp. paratuberculosis.
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Registered trials
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