ArticleApplied and environmental microbiology2009
Importance of polyphosphate kinase 1 for Campylobacter jejuni viable-but-nonculturable cell formation, natural transformation, and antimicrobial resistance.
Article in Applied and environmental microbiology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 57 citations in OpenAlex.
- Roles of viable but non-culturable state in the survival ofFrontiers in cellular and infection microbiology · 2023Article
- Overview of VBNC, a survival strategy for microorganisms.3 Biotech · 2022Review
- Roles of Aerotolerance, Biofilm Formation, and Viable but Non-Culturable State in the Survival ofMicroorganisms · 2022Review
- Endophytic bacterial community diversity in two citrus cultivars with different citrus canker disease resistance.Archives of microbiology · 2021Article
- Molecular Mechanisms of Campylobacter Biofilm Formation and Quorum Sensing.Current topics in microbiology and immunology · 2021Review
- First-time characterization of viable but non-culturable Proteus mirabilis: Induction and resuscitation.Journal of cellular and molecular medicine · 2020Article
- Relationship between the Viable but Nonculturable State and Antibiotic Persister Cells.Journal of bacteriology · 2018Review
- Polyphosphate-Accumulating Bacteria: Potential Contributors to Mineral Dissolution in the Oral Cavity.Applied and environmental microbiology · 2018Article
- Transducer-Like Protein inFrontiers in microbiology · 2018Article
- Effect of divalent ions and a polyphosphate on composition, structure, and stiffness of simulated drinking water biofilms.NPJ biofilms and microbiomes · 2018Article
- Mathematical models of microbial growth and metabolism: a whole-organism perspective.Science progress · 2017Review
- Formate simultaneously reduces oxidase activity and enhances respiration in Campylobacter jejuni.Scientific reports · 2017Article
- Article
- Polyphosphate and associated enzymes as global regulators of stress response and virulence in Campylobacter jejuni.World journal of gastroenterology · 2016Review
- Polyphosphate kinases modulate Campylobacter jejuni outer membrane constituents and alter its capacity to invade and survive in intestinal epithelial cells in vitro.Emerging microbes & infections · 2015Article
- Transducer like proteins of Campylobacter jejuni 81-176: role in chemotaxis and colonization of the chicken gastrointestinal tract.Frontiers in cellular and infection microbiology · 2015Article
- Transcriptome analysis of Campylobacter jejuni polyphosphate kinase (ppk1 and ppk2) mutants.Virulence · 2015Article
- Impact of oxidative stress defense on bacterial survival and morphological change in Campylobacter jejuni under aerobic conditions.Frontiers in microbiology · 2015Article
- Ultrastructure and complex polar architecture of the human pathogen Campylobacter jejuni.MicrobiologyOpen · 2014Article
- Polyphosphate-mediated modulation of Campylobacter jejuni biofilm growth and stability.Virulence · 2014Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Campylobacter jejuni, a gram-negative, microaerophilic bacterium, is a predominant cause of bacterial gastroenteritis in humans. Although considered fragile and fastidious and lacking many classical stress response mechanisms, C. jejuni exhibits a remarkable capacity for survival and adaptation, successfully infecting humans and persisting in the environment. Consequently, understanding the physiological and genetic properties that allow C. jejuni to survive and adapt to various stress conditions is crucial for therapeutic interventions. Of importance is polyphosphate (poly-P) kinase 1 (PPK1), which is a key enzyme mediating the synthesis of poly-P, an essential molecule for survival, mediating stress responses, host colonization, and virulence in many bacteria. Therefore, we investigated the role of PPK1 in C. jejuni pathogenesis, stress survival, and adaptation. Our findings demonstrate that a C. jejuni Deltappk1 mutant was deficient in poly-P accumulation, which was associated with a decreased ability to form viable-but-nonculturable cells under acid stress. The Deltappk1 mutant also showed a decreased frequency of natural transformation and an increased susceptibility to various antimicrobials. Furthermore, the Deltappk1 mutant was characterized by a dose-dependent deficiency in chicken colonization. Complementation of the Deltappk1 mutant with the wild-type copy of ppk1 restored the deficient phenotypes to levels similar to those of the wild type. Our results suggest that poly-P plays an important role in stress survival and adaptation and might contribute to genome plasticity and the spread and development of antimicrobial resistance in C. jejuni. These findings highlight the potential of PPK1 as a novel target for therapeutic interventions.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.